Ben Salem R, Abbassi M S, García V, García-Fierro R, Njoud C, Messadi L, Rodicio M R
Veterinary Research Institute of Tunisia, Tunis, Tunisia.
Faculty of Sciences of Bizert, University of Carthage, Bizert, Tunisia.
Zoonoses Public Health. 2016 Jun;63(4):320-7. doi: 10.1111/zph.12234. Epub 2015 Nov 5.
Salmonella enterica subsp. enterica serovar Eppendorf, with antigenic formula 1,4,12,[27]:d:1,5, is an infrequent serovar. However, 14% (20 of 142) of the isolates recovered during June-July 2012 in chicken farms in Tunisia belonged to S. Eppendorf. These isolates were analysed for resistance and virulence profiles. None of them were susceptible to all antimicrobials tested, while 70%, 60%, 50%, 50%, 20% and 5% were resistant to sulphonamides (sul1, sul2 and sul3), streptomycin (aadA1-like), trimethoprim (dfrA1-like), nalidixic acid (GyrA Asp87 →Asn and not identified), gentamicin (not identified) and ampicillin (blaTEM -1-like). About 30% of the isolates showed decreased susceptibility to ciprofloxacin and carried the qnrB gene; 65% of the isolates were multidrug resistant and contained class 1 integrons with sul1 or sul3 in the 3' conserved segment. The orgA, ssaQ, mgtC, siiD and sopB virulence genes located on SPI1 to SPI5 and the fimbrial bcfC gene were present in all isolates; the sopE1 and sodC1 carried by prophages were variably detected; however, the prophage gipA gene and the spvC gene of serovar-specific virulence plasmids were absent. Altogether, ten resistance and three virulence profiles were identified. Typing of the isolates with XbaI- and BlnI-PFGE supports a close relationship, although they appear to be evolving under selective pressure probably caused by antimicrobial use in chicken husbandry. As far as we know, this is the first study investigating the molecular bases of antimicrobial drug resistance, the virulence gene content and the PFGE profiles of S. Eppendorf. The epidemiological surveillance of this serovar would be necessary to evaluate its possible impact on human health, particularly in Tunisia and other African countries where it was already reported.
肠炎沙门氏菌肠炎亚种埃彭多夫血清型，抗原公式为1,4,12,[27]:d:1,5，是一种罕见的血清型。然而，2012年6月至7月在突尼斯养鸡场分离出的菌株中，14%（142株中的20株）属于埃彭多夫沙门氏菌。对这些分离株进行了耐药性和毒力谱分析。它们中没有一株对所有测试抗菌药物敏感，而70%、60%、50%、50%、20%和5%的菌株分别对磺胺类药物（sul1、sul2和sul3）、链霉素（aadA1样）、甲氧苄啶（dfrA1样）、萘啶酸（GyrA Asp87→Asn且未鉴定）、庆大霉素（未鉴定）和氨苄青霉素（blaTEM -1样）耐药。约30%的分离株对环丙沙星敏感性降低并携带qnrB基因；65%的分离株对多种药物耐药，且在3'保守区段含有带有sul1或sul3的1类整合子。位于SPI1至SPI5上的orgA、ssaQ、mgtC、siiD和sopB毒力基因以及菌毛bcfC基因在所有分离株中均存在；噬菌体携带的sopE1和sodC1检测结果不一；然而，噬菌体gipA基因和血清型特异性毒力质粒的spvC基因缺失。总共鉴定出十种耐药谱和三种毒力谱。用XbaI和BlnI - PFGE对分离株进行分型支持它们之间存在密切关系，尽管它们似乎在可能由养鸡业中抗菌药物使用导致的选择压力下进化。据我们所知，这是第一项研究埃彭多夫沙门氏菌抗菌药物耐药性的分子基础、毒力基因含量和PFGE图谱的研究。对该血清型进行流行病学监测对于评估其对人类健康的可能影响是必要的，特别是在突尼斯和其他已报告该血清型的非洲国家。
