Ben Salem Rakia, Abbassi Mohamed S, García Vanesa, García-Fierro Raquel, Fernández Javier, Kilani Hajer, Jaouani Imen, Khayeche Monia, Messadi Lilia, Rodicio María R
Veterinary Research Institute of Tunisia, 20 Street Jebel Lakhdhar, Bab Saadoun, 1006 Tunis, Tunisia; Faculté des Sciences de Bizerte, Université de Carthage, Tunis, Tunisia.
Veterinary Research Institute of Tunisia, 20 Street Jebel Lakhdhar, Bab Saadoun, 1006 Tunis, Tunisia; Faculté des Sciences de Bizerte, Université de Carthage, Tunis, Tunisia.
J Infect Public Health. 2017 Nov-Dec;10(6):855-860. doi: 10.1016/j.jiph.2017.01.012. Epub 2017 Feb 16.
This study focused on 77 isolates of Salmonella enterica serotype Enteritidis collected during 2009 to 2013 from healthy and sick chickens and environmental farm samples in Tunisia. Resistance to 14 antimicrobials and the encoding genes were analyzed. 66, 26, 6.5, 3.9 and 1.3% were pan-susceptible or showed resistance to nalidixic acid (Asp87 to Tyr and Asp87 to Asn substitutions in GyrA), ampicillin (bla and bla), sulfonamides (sul1and sul3) and streptomycin (strB), respectively. A single isolate with intermediate susceptibility to ciprofloxacin was positive for qnrB, whereas qnrA, qnrS or aac(6')-Ib-cr were not detected. The virulotype of the isolates was established by testing ten virulence genes. The orgA, ssaQ, mgtC, siiD, sopB genes, located on Salmonella pathogenicity islands, and spvC of the serotype-specific virulence plasmid, were common to all isolates. In contrast, the prophage-associated sopE-1, sodC1 and gipA genes and the fimbrial bcfC gene were variably represented. All isolates except one contained the virulence plasmid, which appeared either alone or together with one or more additional plasmids. One isolate carried a single plasmid of ca. 90Kb which may be derived from the virulence plasmid (60Kb). Overall, seven resistotypes, six virulotypes and six plasmid profiles were identified. XbaI-PFGE revealed four related pulsotypes (X1-X4), with 80% of the isolates sharing the X1 pattern. The latter isolates exhibited different resistance, virulence and plasmid profiles, suggesting that mobile genetic elements, particularly prophages and plasmids, are of central importance for the evolution and adaptation of S. Enteritidis circulating in chicken farms in Tunisia.
本研究聚焦于2009年至2013年期间从突尼斯健康和患病鸡以及农场环境样本中收集的77株肠炎沙门氏菌肠炎血清型菌株。分析了这些菌株对14种抗菌药物的耐药性及其编码基因。分别有66%、26%、6.5%、3.9%和1.3%的菌株对所有测试抗菌药物敏感或对萘啶酸（GyrA中Asp87突变为Tyr以及Asp87突变为Asn）、氨苄西林（bla和bla）、磺胺类药物（sul1和sul3）以及链霉素（strB）耐药。一株对环丙沙星中度敏感的菌株qnrB呈阳性，而未检测到qnrA、qnrS或aac(6')-Ib-cr。通过检测十个毒力基因确定了菌株的毒力型。位于沙门氏菌致病岛的orgA、ssaQ、mgtC、siiD、sopB基因以及血清型特异性毒力质粒的spvC基因在所有菌株中均存在。相反，噬菌体相关的sopE-1、sodC1和gipA基因以及菌毛bcfC基因的存在情况各不相同。除一株菌株外，所有菌株都含有毒力质粒，该质粒可单独出现或与一个或多个其他质粒共同出现。一株菌株携带一个约90Kb的单一质粒，该质粒可能源自毒力质粒（60Kb）。总体而言，鉴定出了七种耐药型、六种毒力型和六种质粒图谱。XbaI-PFGE显示出四种相关的脉冲型（X1 - X4），80%的菌株具有X1图谱。后一组菌株表现出不同的耐药性、毒力和质粒图谱，这表明移动遗传元件，特别是噬菌体和质粒，对于突尼斯养鸡场中传播的肠炎沙门氏菌的进化和适应性至关重要。
