Di Cerbo A, Palatucci A T, Rubino V, Centenaro S, Giovazzino A, Fraccaroli E, Cortese L, Ruggiero G, Guidetti G, Canello S, Terrazzano G
School of Specialization in Clinical Biochemistry, "G. d'Annunzio" University, Chieti, Italy.
PhD School of Science, University of Basilicata, 85100, Potenza, Italy.
J Biochem Mol Toxicol. 2016 Apr;30(4):170-7. doi: 10.1002/jbt.21775. Epub 2015 Nov 5.
Antibiotics are widely used in zoo technical and veterinary practices as feed supplementation to ensure wellness of farmed animals and livestock. Several evidences have been suggesting both the toxic role for tetracyclines, particularly for oxytetracycline (OTC). This potential toxicity appears of great relevance for human nutrition and for domestic animals. This study aimed to extend the evaluation of such toxicity. The biologic impact of the drug was assessed by evaluating the proinflammatory effect of OTC and their bone residues on cytokine secretion by in vitro human peripheral blood lymphocytes. Our results showed that both OTC and OTC-bone residues significantly induced the T lymphocyte and non-T cell secretion of interferon (IFN)-γ, as cytokine involved in inflammatory responses in humans as well as in animals. These results may suggest a possible implication for new potential human and animal health risks depending on the entry of tetracyclines in the food-processing chain.
抗生素在动物饲养技术和兽医实践中被广泛用作饲料添加剂，以确保养殖动物和家畜的健康。有多项证据表明四环素，尤其是土霉素（OTC）具有毒性作用。这种潜在毒性对人类营养和家畜似乎具有重大意义。本研究旨在扩大对这种毒性的评估。通过评估OTC及其骨残留对体外培养的人外周血淋巴细胞分泌细胞因子的促炎作用，来评估该药物的生物学影响。我们的结果表明，OTC及其骨残留均显著诱导T淋巴细胞和非T细胞分泌干扰素（IFN）-γ，IFN-γ是参与人类和动物炎症反应的细胞因子。这些结果可能表明，由于四环素进入食品加工链，可能会带来新的潜在人类和动物健康风险。
