Chi Zhenxing, Liu Rutao, You Hong, Ma Shanshan, Cui Hao, Zhang Qiang
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, PR China; School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, PR China.
School of Environmental Science and Engineering, Shandong University, Jinan, PR China.
PLoS One. 2014 Jul 14;9(7):e102334. doi: 10.1371/journal.pone.0102334. eCollection 2014.
The study investigated the effect of oxytetracycline (OTC) on the anti-oxidative defense system, the structure (hemolysis rate and morphology) and function (ATP enzyme activity) of human red blood cells (hRBCs) to investigate the possible toxic mechanism of OTC to hRBCs. The experimental results indicate that OTC can cause a decline in the function of the antioxidant defense system of hRBCs, resulting in oxidative stress. OTC can bring about morphological changes to hRBCs, and further leads to hemolysis, when the concentration of OTC is over 8×10(-5) M (about 164 µg/ml). At a low OTC concentration, below 4×10(-5) M (82 µg/ml), OTC can enhance the activity of ATP enzyme of hRBCs, known as hormesis. However, at a high concentration, above 4×10(-5) M (about 82 µg/ml), the ATP enzymatic activity was inhibited, affecting the function of hRBCs. The estalished mechanism of toxicity of OTC to hRBCs can facilitate a deeper understanding of the toxicity of OTC in vivo.
该研究调查了土霉素（OTC）对人红细胞（hRBCs）抗氧化防御系统、结构（溶血率和形态）及功能（ATP酶活性）的影响，以探究OTC对hRBCs可能的毒性机制。实验结果表明，OTC可导致hRBCs抗氧化防御系统功能下降，从而产生氧化应激。当OTC浓度超过8×10⁻⁵ M（约164 µg/ml）时，OTC可使hRBCs发生形态变化，并进一步导致溶血。在低OTC浓度下，即低于4×10⁻⁵ M（82 µg/ml）时，OTC可增强hRBCs的ATP酶活性，即表现为兴奋效应。然而，在高浓度下，即高于4×10⁻⁵ M（约82 µg/ml）时，ATP酶活性受到抑制，影响hRBCs的功能。所建立的OTC对hRBCs的毒性机制有助于更深入地了解OTC在体内的毒性。
