通过体内31P-NMR观察2-脱氧葡萄糖对酿酒酵母的影响。
Effects of 2-deoxyglucose on Saccharomyces cerevisiae as observed by in vivo 31P-NMR.
作者信息
Loureiro-Dias M C, Santos H
机构信息
Laboratory of Microbiology, Gulbenkian Institute of Science, Oeiras, Portugal.
出版信息
FEMS Microbiol Lett. 1989 Jan 1;48(1):25-8. doi: 10.1016/0378-1097(89)90140-7.
Saccharomyces cerevisiae cells were treated with 2-deoxyglucose (1 mM) and the effects induced in the levels of phosphorus compounds and in the internal pH were monitored using 31P-NMR. Upon incubation with 2-deoxyglucose a strong decrease in the polyphosphate level was observed and the cytoplasmic pH decreased by about 0.4 units. This shows that 2-deoxyglucose strongly interferes with the cell conditions and consequently, the results of experiments in which 2-deoxyglucose was used to obtain deenergized cells should be carefully reanalysed.
用2-脱氧葡萄糖（1 mM）处理酿酒酵母细胞，并使用31P-NMR监测磷化合物水平和细胞内pH值的变化。与2-脱氧葡萄糖孵育后，观察到多聚磷酸盐水平显著下降，细胞质pH值下降约0.4个单位。这表明2-脱氧葡萄糖强烈干扰细胞状态，因此，对于使用2-脱氧葡萄糖来获得能量耗尽细胞的实验结果，应仔细重新分析。
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