Shi Huizhen, Zhang Shuyi, Mao Xiuguang
a Institute of Molecular Ecology and Evolution, SKLEC, East China Normal University, Shanghai , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4545-4546. doi: 10.3109/19401736.2015.1101555. Epub 2015 Nov 5.
The king horseshoe bat (Rhinolophus rex) is endemic to China. Here, we characterized the complete mitochondrial genome of R. rex using next-generation sequencing and Sanger sequencing. The mitogenome is 16 845 bp in length and contains 22 tRNAs, two rRNAs, 13 protein-coding genes, and a non-coding control region. Our traditional Sanger sequencing verified that mtDNA sequences generated from next-generation sequencing are reliable. The mitogenome of R. rex will be useful for the conservation genetics of this endangered species.
中菊头蝠（Rhinolophus rex）是中国特有的物种。在此，我们利用二代测序和桑格测序对中菊头蝠的完整线粒体基因组进行了特征分析。该线粒体基因组长度为16845bp，包含22个tRNA、两个rRNA、13个蛋白质编码基因和一个非编码控制区。我们的传统桑格测序验证了二代测序产生的mtDNA序列是可靠的。中菊头蝠的线粒体基因组将有助于该濒危物种的保护遗传学研究。
