甲基化对精氨酸侧链pKa值的影响。
Effect of methylation on the side-chain pKa value of arginine.
作者信息
Evich Marina, Stroeva Ekaterina, Zheng Yujun George, Germann Markus W
机构信息
Department of Chemistry, Georgia State University, Atlanta, Georgia, 30303.
Department of Chemistry, University of Georgia, Athens, Georgia, 30602.
出版信息
Protein Sci. 2016 Feb;25(2):479-86. doi: 10.1002/pro.2838. Epub 2015 Nov 25.
Abstract
Arginine methylation is important in biological systems. Recent studies link the deregulation of protein arginine methyltransferases with certain cancers. To assess the impact of methylation on interaction with other biomolecules, the pKa values of methylated arginine variants were determined using NMR data. The pKa values of monomethylated, symmetrically dimethylated, and asymmetrically dimethylated arginine are similar to the unmodified arginine (14.2 ± 0.4). Although the pKa value has not been significantly affected by methylation, consequences of methylation include changes in charge distribution and steric effects, suggesting alternative mechanisms for recognition.
摘要
精氨酸甲基化在生物系统中很重要。最近的研究将蛋白质精氨酸甲基转移酶的失调与某些癌症联系起来。为了评估甲基化对与其他生物分子相互作用的影响，使用核磁共振（NMR）数据测定了甲基化精氨酸变体的pKa值。单甲基化、对称二甲基化和不对称二甲基化精氨酸的pKa值与未修饰的精氨酸相似（14.2±0.4）。虽然pKa值没有受到甲基化的显著影响，但甲基化的后果包括电荷分布和空间效应的变化，这表明存在其他识别机制。
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