Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Antibody Discovery and Protein Engineering, MedImmune, Gaithersburg, MD 20878, USA.
Curr Opin Biotechnol. 2015 Dec;36:215-21. doi: 10.1016/j.copbio.2015.09.006. Epub 2015 Nov 2.
Protein secretion and vesicular trafficking in mammalian cells rely on several key lipids including sphingolipids, phospholipids, and neutral lipids crucial to protein processing and other intracellular events. Proteins interact with these lipids to alter the shape of lipid bilayer, thereby playing a pivotal role in cellular sorting. Although some efforts have elucidated the role of these components, extensive studies are needed to further decipher the protein-lipid interactions along with the effect of membrane curvature and rafts in sorting of proteins. The regulatory role of proteins in subcellular localization and metabolism of lipids also needs to be described. Recent studies on the role of lipid-protein interactions in modulating membrane shape, signal transduction, and vesicular trafficking are presented in this review.
哺乳动物细胞中的蛋白质分泌和囊泡运输依赖于几种关键脂质，包括鞘脂、磷脂和中性脂质，这些脂质对蛋白质加工和其他细胞内事件至关重要。蛋白质与这些脂质相互作用以改变脂质双层的形状，从而在细胞分拣中发挥关键作用。尽管已经有一些努力阐明了这些成分的作用，但仍需要广泛的研究来进一步破译蛋白质-脂质相互作用以及膜曲率和筏在蛋白质分拣中的作用。蛋白质在脂质亚细胞定位和代谢中的调节作用也需要描述。本文综述了脂质-蛋白质相互作用在调节膜形状、信号转导和囊泡运输中的作用的最新研究进展。
