Moriya Hisao
Research Core for Interdisciplinary Sciences, Okayama University, Okayama 700-8530, Japan
Mol Biol Cell. 2015 Nov 5;26(22):3932-9. doi: 10.1091/mbc.E15-07-0512.
Overexpression experiments are sometimes considered as qualitative experiments designed to identify novel proteins and study their function. However, in order to draw conclusions regarding protein overexpression through association analyses using large-scale biological data sets, we need to recognize the quantitative nature of overexpression experiments. Here I discuss the quantitative features of two different types of overexpression experiment: absolute and relative. I also introduce the four primary mechanisms involved in growth defects caused by protein overexpression: resource overload, stoichiometric imbalance, promiscuous interactions, and pathway modulation associated with the degree of overexpression.
过表达实验有时被视为旨在鉴定新蛋白质并研究其功能的定性实验。然而，为了通过使用大规模生物数据集的关联分析得出关于蛋白质过表达的结论，我们需要认识到过表达实验的定量性质。在此，我将讨论两种不同类型过表达实验的定量特征：绝对过表达和相对过表达。我还将介绍蛋白质过表达导致生长缺陷所涉及的四种主要机制：资源过载、化学计量失衡、混杂相互作用以及与过表达程度相关的途径调节。
