Tassew Zebasil, Chandravanshi Bhagwan Singh
Quality Control Department, Ethiopian Pharmaceuticals Manufacturing Sh. Co. (Epharm), P.O. Box 2457, Addis Ababa, Ethiopia ; Department of Chemistry, Bahir Dar University, P.O. Box 79 Bahir Dar, Ethiopia.
Department of Chemistry, College of Natural Sciences, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia.
Springerplus. 2015 Oct 29;4:649. doi: 10.1186/s40064-015-1448-y. eCollection 2015.
Tobacco is a valuable cash crop. It is the most widely grown non-food crop in the world. Tobacco use is widespread due to its addictive nature of its main constituent nicotine. Therefore, the knowledge of nicotine level in tobacco is important to tobacco industry and in the area of toxicology to control its harmful effect on health. There is no report in the literature on nicotine level of Ethiopian raw (unprocessed) tobacco leaves. Hence, the objective of this study is to determine the levels of nicotine in the Ethiopian tobacco leaves. Samples were collected based on their leaves positions, species and place of cultivation from different regions of Ethiopia. These were Virginia type tobacco from Shewa Robit and Billate, Burley and Oriental types of tobacco from Awassa and native tobacco used as pipe smoking (Gaya) from Wollayita. The level of nicotine in four different varieties of Ethiopian tobacco leaves was determined using high performance liquid chromatography. The level of nicotine in the four different varieties of Ethiopian tobacco were Virginia tobacco (3.26 %), the native tobacco 'Gaya' (1.10 %), Burley tobacco (0.650 %), and Oriental tobacco leaves (≤0.0500 %). It was found that the nicotine level of Ethiopian Virginia tobacco leaves increases from bottom to top leaf (stalk) positions of the tobacco plant. It was also found that the nicotine level of Ethiopian tobacco leaves varies in different species and the nicotine level of the same tobacco species differ in different area of cultivation. In general, the level of nicotine in Ethiopian tobacco is comparable with that in the rest of the world.
烟草是一种重要的经济作物。它是世界上种植最广泛的非粮食作物。由于其主要成分尼古丁具有成瘾性，烟草的使用非常普遍。因此，了解烟草中的尼古丁含量对于烟草行业以及毒理学领域控制其对健康的有害影响至关重要。文献中没有关于埃塞俄比亚生（未加工）烟叶尼古丁含量的报道。因此，本研究的目的是测定埃塞俄比亚烟叶中的尼古丁含量。根据烟叶的位置、品种和种植地点，从埃塞俄比亚不同地区采集样本。这些样本包括来自谢瓦·罗比特和比拉特的弗吉尼亚型烟草、来自阿瓦萨的白肋烟和东方型烟草，以及来自沃拉伊塔用作烟斗吸烟的本地烟草（加亚）。使用高效液相色谱法测定了埃塞俄比亚四种不同品种烟叶中的尼古丁含量。埃塞俄比亚四种不同品种烟叶中的尼古丁含量分别为：弗吉尼亚烟草（3.26%）、本地烟草“加亚”（1.10%）、白肋烟（0.650%）和东方型烟叶（≤0.0500%）。研究发现，埃塞俄比亚弗吉尼亚烟叶中的尼古丁含量从烟草植株底部到顶部叶片（茎）位置逐渐增加。还发现埃塞俄比亚烟叶中的尼古丁含量因品种不同而有所差异，同一烟草品种在不同种植地区的尼古丁含量也不同。总体而言，埃塞俄比亚烟草中的尼古丁含量与世界其他地区相当。
