Tobacco College of Henan Agricultural University, No. 95, Wenhua Road, Zhengzhou, Henan Province, 450002, P.R. China.
Luoyang Tobacco Company, No. 246, Kaiyuan Road, Luoyang, Henan Province, 471000, P.R. China.
Sci Rep. 2017 Dec 12;7(1):17442. doi: 10.1038/s41598-017-17045-2.
This study aimed to investigate drought resistance of the LY1306 tobacco strain. Seedlings of tobacco strains LY1306, ZhongYan 100 (ZY100) and Hong Hua Da Jin Yuan (HHDJY) were treated with polyethylene glycol (PEG)-6000 to induce osmotic stress. As validation, water-deficit-induced drought treatments, including mild drought (MD; watering 1.5 L/week) and severe drought (SD, without watering) were carried out. Changes in cell morphology, leaf water potential, antioxidant enzyme activity, as well as contents of malondialdehyde (MDA) and proline were determined for each treatment. Transcriptome sequencing was performed for the seedlings treated with 15% PEG-6000. No obvious changes were observed in morphology of LY1306 and ZY100 under osmotic or drought stress; whereas, visible wilting was observed in HHDJY. Superoxide dismutase and peroxidase activities of LY1036 and ZY100 under osmotic stress were significantly higher than those of HHDJY. Under SD, the MDA content of LY1306 was significantly lower and the proline content of LY1306 was significantly higher than that of HHDJY. Differential genes between LY1306, ZY100 and HHDJY were enriched in functions about alpha-linolenic acid, and arginine and proline metabolisms. LY1306 could increase its antioxidant enzyme activities and proline accumulation in response to drought stress, probably by regulating drought resistance-related pathways and genes.
本研究旨在探究 LY1306 烟草品系的耐旱性。使用聚乙二醇（PEG）-6000 处理烟草品系 LY1306、中烟 100（ZY100）和红花大金元（HHDJY）的幼苗，以诱导渗透胁迫。作为验证，对幼苗进行水分亏缺诱导的干旱处理，包括轻度干旱（MD，每周浇水 1.5L）和重度干旱（SD，不浇水）。对每种处理的细胞形态、叶片水势、抗氧化酶活性以及丙二醛（MDA）和脯氨酸的含量变化进行了测定。对用 15%PEG-6000 处理的幼苗进行了转录组测序。在渗透或干旱胁迫下，LY1306 和 ZY100 的形态没有明显变化；而 HHDJY 则出现明显萎蔫。LY1036 和 ZY100 在渗透胁迫下的超氧化物歧化酶和过氧化物酶活性明显高于 HHDJY。在 SD 下，LY1306 的 MDA 含量显著降低，脯氨酸含量显著高于 HHDJY。LY1306、ZY100 和 HHDJY 之间的差异基因在α-亚麻酸和精氨酸及脯氨酸代谢相关功能上富集。LY1306 可能通过调节与耐旱性相关的途径和基因，增加其抗氧化酶活性和脯氨酸积累，从而应对干旱胁迫。
