Espe M, Holen E, He J, Provan F, Chen L, Øysæd K B, Seliussen J
National Institute of Nutrition and Seafood Research (NIFES), PoBox 2029, 5817 Nordnes, Norway.
National Institute of Nutrition and Seafood Research (NIFES), PoBox 2029, 5817 Nordnes, Norway ; Fish Nutrition Laboratory, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China.
Springerplus. 2015 Oct 31;4:658. doi: 10.1186/s40064-015-1432-6. eCollection 2015.
Hydrolyzed fish proteins (H-pro) contains high concentrations of free amino acids and low molecular peptides that potentially benefit health. The following study aimed to test whether the water soluble phase of H-pro could reduce apoptosis and inflammation in primary liver cells isolated from Atlantic salmon following H2O2 provoked oxidative stress. Cells were grown as monocultures or co-cultured with head kidney cells to assess possible cross talk in inflammation and metabolism during treatments. Cells were grown in media with or without H-pro for 2 days before being stressed with 200 µM H2O2 then harvested 24 h post exposure. Both treatments were compared to the respective treatments without H2O2 supplementation. Oxidative stressed cells had increased activation of caspase-3, but supplementation with H-pro in the media prior to the oxidative stress reduced caspase-3 activation. In conclusion, free amino acids and low molecular weight peptides from H-pro attenuated oxidative stress, and made cells able to withstand apoptosis after H2O2 provoked oxidative stress.
水解鱼蛋白（H-pro）含有高浓度的游离氨基酸和低分子肽，可能对健康有益。以下研究旨在测试H-pro的水溶性部分是否能减少过氧化氢引发氧化应激后从大西洋鲑鱼分离的原代肝细胞中的细胞凋亡和炎症。细胞以单培养或与头肾细胞共培养的方式生长，以评估处理过程中炎症和代谢方面可能存在的相互作用。细胞在含或不含H-pro的培养基中培养2天，然后用200μM过氧化氢进行应激处理，暴露后24小时收获。将这两种处理与各自不添加过氧化氢的处理进行比较。氧化应激细胞中caspase-3的激活增加，但在氧化应激之前在培养基中添加H-pro可降低caspase-3的激活。总之，H-pro中的游离氨基酸和低分子量肽减轻了氧化应激，并使细胞能够在过氧化氢引发氧化应激后抵抗细胞凋亡。
