Jin Soo-Young, Kim Su-Gwan, Oh Ji-Su, You Jae-Seek, Lim Sung-Chul, Jeong Mi-Ae, Kim Jin-Son
*Graduate Student, Department of Oral and Maxillofacial Surgery, College of Dentistry, Chosun University, Gwangju, Republic of Korea. †Professor, Department of Oral and Maxillofacial Surgery, School of Dentistry, Chosun University, Gwangju, Republic of Korea. ‡Associate Professor, Department of Oral and Maxillofacial Surgery, School of Dentistry, Chosun University, Gwangju, Republic of Korea. §Assistant Professor, Department of Oral and Maxillofacial Surgery, School of Dentistry, Chosun University, Gwangju, Republic of Korea. ¶Professor, Department of Pathology, School of Medicine, Chosun University, Gwangju, Republic of Korea. ‖Associate Professor, Department of Dental Hygiene, Kangwon National University, Samcheok, Republic of Korea. #Clinical Doctor, Misomore Dental Clinic, Jeonju, Republic of Korea.
Implant Dent. 2016 Feb;25(1):83-9. doi: 10.1097/ID.0000000000000351.
The purpose of this study was to evaluate histomorphometrically contaminated autogenous tooth graft materials, which were resterilized.
The intentional defects (diameter: 8 mm, depth: 4 mm) were formed around implant fixture on the iliac crest of 6 mongrel dogs. Autogenous tooth graft materials were made by extracted premolars. After the contamination of the tooth materials, graft procedure was performed; no contaminated group (control group), contaminated groups (nonsterilization group [group 1], ethylene oxide [EO] gas group [group 2], and autoclave group [group 3]). The bone-to-implant contact (BIC) and the new bone formation rate (NBFR) were evaluated after sacrifice.
The BIC and NBFR of groups 1 and 3 were significantly lower than the control group after 4 weeks. The BIC and NBRF of group 3 were significantly lower than the control group after 8 weeks. However, the BIC and NBRF of group 2 was not significantly different comparing with the control group after 4 and 8 weeks.
Sterilization using EO gas may be more favorable than high-pressure sterilization in cases the reuse of contaminated autogenous tooth graft materials.
本研究旨在通过组织形态计量学评估经再次灭菌的受污染自体牙移植材料。
在6只杂种犬的髂嵴上围绕种植体形成有意的缺损（直径：8毫米，深度：4毫米）。自体牙移植材料由拔除的前磨牙制成。牙材料污染后进行移植手术；分为未污染组（对照组）、污染组（未灭菌组[第1组]、环氧乙烷[EO]气体组[第2组]和高压灭菌组[第3组]）。处死动物后评估骨与种植体接触（BIC）和新骨形成率（NBFR）。
4周后，第1组和第3组的BIC和NBFR显著低于对照组。8周后，第3组的BIC和NBRF显著低于对照组。然而，4周和8周后，第2组的BIC和NBRF与对照组相比无显著差异。
在受污染的自体牙移植材料再利用的情况下，使用环氧乙烷气体灭菌可能比高压灭菌更有利。
