Yoshida A, Fukazawa M, Ushio H, Aiyoshi Y, Soeda S, Ito K
Department of Surgery, University of Tsukuba, Ibaraki, Japan.
J Surg Oncol. 1989 May;41(1):1-4. doi: 10.1002/jso.2930410104.
The cell kinetics of the transplantable human anaplastic thyroid carcinoma were studied in athymic nude mice using bromodeoxyuridine (BrdU) pulse labeling. In order to estimate the cell cycle time in this study, the percent labeled mitosis curve was prepared with immunoperoxidase staining using monoclonal antibody to BrdU instead of 3[H]thymidine autoradiography. This technique was considered to be very useful because it can be done for a short term and can still be changed to the autoradiographic method. Comparing the results in this study with already reported data on various xenografts of human carcinomas in nude mice, the tumor doubling time (117.5 hr) and the cell cycle time (23.5 hr) were rather short, but the growth fraction (54%) and the cell loss factor (68%) were nearly equivalent to the median value. These findings suggest that the very rapid growth rate of this tumor was mainly due to the shortness of the cell cycle time.
利用溴脱氧尿苷（BrdU）脉冲标记法，在无胸腺裸鼠中研究了可移植性人未分化甲状腺癌的细胞动力学。为了在本研究中估算细胞周期时间，采用抗BrdU单克隆抗体通过免疫过氧化物酶染色制备标记有丝分裂百分数曲线，而非采用³H胸腺嘧啶核苷放射自显影法。该技术被认为非常有用，因为它可在短期内完成，且仍可转换为放射自显影法。将本研究结果与已报道的关于裸鼠中人癌各种异种移植的数据进行比较，肿瘤倍增时间（117.5小时）和细胞周期时间（23.5小时）相当短，但生长分数（54%）和细胞丢失率（68%）几乎等同于中位数。这些发现表明，该肿瘤非常快的生长速率主要归因于细胞周期时间较短。
