Kikuyama S, Kubota T, Watanabe M, Isobe Y, Fukutomi T, Ishibiki K, Abe O
Jpn J Surg. 1987 Jan;17(1):28-32. doi: 10.1007/BF02470581.
The cell kinetics of human tumor xenografts serially transplanted into nude mice was examined using bromodeoxyuridine (BrdU) and anti-BrdU monoclonal antibody. After various doses of BrdU were given intraperitoneally into tumor bearing nude mice, the tumors were resected and stained immunohistochemically, using the anti-BrdU monoclonal antibody. The number of stained cells was demonstrated as the labeling index (LI). The optimal condition for BrdU staining was assumed to be 300 mg of BrdU per kg followed by an incubation period of one hour. Since the LI by BrdU closely correlated with that in autoradiography by 3H-TdR, this method is more useful and safer than the conventional autoradiographic study for investigating clinical cell kinetic analysis, as there is no need to use potentially hazardous radioactive compounds and the period of assay is shorter.
使用溴脱氧尿苷（BrdU）和抗BrdU单克隆抗体检测了连续移植到裸鼠体内的人肿瘤异种移植物的细胞动力学。向荷瘤裸鼠腹腔注射不同剂量的BrdU后，切除肿瘤并用抗BrdU单克隆抗体进行免疫组织化学染色。染色细胞的数量被作为标记指数（LI）。BrdU染色的最佳条件被认为是每千克300毫克BrdU，随后孵育1小时。由于BrdU的LI与3H-TdR放射自显影的LI密切相关，因此该方法比传统的放射自显影研究更有用、更安全，可用于临床细胞动力学分析，因为无需使用潜在危险的放射性化合物，且检测周期更短。
