Lee Jung-Ah, Lee Nak-Hyung, Lee Joong-Bok, Park Seung-Yong, Song Chang-Seon, Choi In-Soo, Lee Sang-Won
College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea; Division of Vaccine Research, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Osong-eup, CheongJu, Chungcheongbuk-do 363-951, Republic of Korea.
College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
Infect Genet Evol. 2016 Jun;40:288-294. doi: 10.1016/j.meegid.2015.11.001. Epub 2015 Nov 3.
Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically significant diseases in the swine industry. The PRRS virus (PRRSV) has genetically diverse populations, like other RNA viruses, and various field strains continue to be reported worldwide. The molecular epidemiological study of PRRSV can provide important data for use in controlling the disease. In this study, 50 oral fluid samples from conventional farms in Korea were taken to analyze nucleotide sequences of the open reading frame 5 of PRRSV. The viruses present in more than 80% of oral fluid samples genetically originated from the type 2 PRRSV, which is North American (NA) lineage. In addition 8.9% of samples contained both of the type 1 PRRSV, which is European (EU) lineage and the type 2 PRRSV. About 60% of farms involved in this study had more than two strains of PRRSV. In phylogenetic analysis, the Korean field strains of PRRSV detected from the oral fluid samples were divided into several subgroups: four subgroups of Korean field strains clustered with the type 1 PRRSV, and other five subgroups of Korean field strains clustered with the type 2. These results suggest that the type 2 PRRSV is more prevalent than the type 1 in Korea and heterologous strains of PRRSV can simultaneously infect a single pig farm.
猪繁殖与呼吸综合征（PRRS）是养猪业中经济影响最为重大的疾病之一。与其他RNA病毒一样，PRRS病毒（PRRSV）具有基因多样的群体，世界各地仍不断有各种田间毒株被报道。PRRSV的分子流行病学研究可为疾病防控提供重要数据。在本研究中，采集了韩国传统猪场的50份口腔液体样本，以分析PRRSV开放阅读框5的核苷酸序列。超过80%的口腔液体样本中存在的病毒在基因上源自2型PRRSV，即北美（NA）谱系。此外，8.9%的样本同时含有1型PRRSV（欧洲（EU）谱系）和2型PRRSV。参与本研究的猪场中约60%有两种以上的PRRSV毒株。在系统发育分析中，从口腔液体样本中检测到的韩国PRRSV田间毒株被分为几个亚组：四个韩国田间毒株亚组与1型PRRSV聚类，其他五个韩国田间毒株亚组与2型聚类。这些结果表明，在韩国2型PRRSV比1型更普遍，并且PRRSV的异源毒株可同时感染单个猪场。
