Yoon S H, Song J-Y, Lee C-H, Choi E-J, Cho I S, Kim B
Virology Division, National Veterinary Research and Quarantine Service, 480, Anyang 6-dong, Anyang, Gyeonggido, Republic of Korea.
Arch Virol. 2008;153(4):627-35. doi: 10.1007/s00705-007-0027-0. Epub 2008 Jan 29.
To investigate the genetic characteristics of the Korean porcine reproductive and respiratory syndrome virus (PRRSV), we determined the complete sequence of the nucleocapsid protein gene (ORF7) from 105 PRRSV isolates from all nine Korean prefectures during the years 2003 through 2006. These sequences were then analyzed along with the published ORF7 sequences for two Korean PRRS viruses (PL97-1/1997 and LMY/2002) and 36 non-Korean viruses. The ORF7 nucleotide sequence identities among the 107 Korean PRRS viruses ranged from 86.2 to 100%, corresponding to 85.4 to 100% identity at the amino acid level. All of the Korean isolates examined belonged to the North American genotype. The ORF7 gene sequence from the North American prototype virus (VR-2332) and its derived vaccine virus (Ingelvac PRRS MLV) was 90.0-100% identical to the various ORF7 sequences of the Korean isolates, with corresponding amino acid identities from 91.0 to 100%. In the phylogenetic tree obtained by neighbor-joining analysis, all of the Korean PRRSVs were divided into four groups. Our ORF7 sequence data also revealed no correlations between the date or place of collection and the distribution of PRRSV in Korea. North American genotype PRRSVs may have been introduced into Korean swine herds some time ago; these viruses apparently radiated nationwide within a relatively short period of time. Within the North American genotype PRRSVs from around the world, the Korean PRRSVs did not emerge as a single independent clade overall, and their immediate relationships with the PRRSVs from other countries could not be determined.
为了研究韩国猪繁殖与呼吸综合征病毒（PRRSV）的遗传特征，我们测定了2003年至2006年期间从韩国九个道采集的105株PRRSV分离株的核衣壳蛋白基因（ORF7）的完整序列。然后将这些序列与已发表的两种韩国PRRS病毒（PL97 - 1/1997和LMY/2002）以及36种非韩国病毒的ORF7序列进行分析。107株韩国PRRS病毒的ORF7核苷酸序列同一性范围为86.2%至100%，氨基酸水平的同一性对应为85.4%至100%。所有检测的韩国分离株均属于北美基因型。北美原型病毒（VR - 2332）及其衍生疫苗病毒（Ingelvac PRRS MLV）的ORF7基因序列与韩国分离株的各种ORF7序列有90.0% - 100%的同一性，相应的氨基酸同一性为91.0%至100%。在通过邻接法分析得到的系统发育树中，所有韩国PRRSV被分为四组。我们的ORF7序列数据还显示，采集日期或地点与韩国PRRSV的分布之间没有相关性。北美基因型PRRSV可能在一段时间前被引入韩国猪群；这些病毒显然在相对较短的时间内传播到了全国。在来自世界各地的北美基因型PRRSV中，韩国PRRSV总体上并未形成一个单一的独立分支，并且无法确定它们与其他国家PRRSV的直接关系。
