Gómez-Molero Emilia, de Boer Albert D, Dekker Henk L, Moreno-Martínez Ana, Kraneveld Eef A, Chauhan Neeraj, Weig Michael, de Soet Johannes J, de Koster Chris G, Bader Oliver, de Groot Piet W J
Regional Center for Biomedical Research, Albacete Science & Technology Park, University of Castilla-La Mancha, E-02008 Albacete, Spain Institute for Medical Microbiology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany.
Regional Center for Biomedical Research, Albacete Science & Technology Park, University of Castilla-La Mancha, E-02008 Albacete, Spain.
FEMS Yeast Res. 2015 Dec;15(8). doi: 10.1093/femsyr/fov098. Epub 2015 Nov 5.
Attachment to human host tissues or abiotic medical devices is a key step in the development of infections by Candida glabrata. The genome of this pathogenic yeast codes for a large number of adhesins, but proteomic work using reference strains has shown incorporation of only few adhesins in the cell wall. By making inventories of the wall proteomes of hyperadhesive clinical isolates and reference strain CBS138 using mass spectrometry, we describe the cell wall proteome of C. glabrata and tested the hypothesis that hyperadhesive isolates display differential incorporation of adhesins. Two clinical strains (PEU382 and PEU427) were selected, which both were hyperadhesive to polystyrene and showed high surface hydrophobicity. Cell wall proteome analysis under biofilm-forming conditions identified a core proteome of about 20 proteins present in all C. glabrata strains. In addition, 12 adhesin-like wall proteins were identified in the hyperadherent strains, including six novel adhesins (Awp8-13) of which only Awp12 was also present in CBS138. We conclude that the hyperadhesive capacity of these two clinical C. glabrata isolates is correlated with increased and differential incorporation of cell wall adhesins. Future studies should elucidate the role of the identified proteins in the establishment of C. glabrata infections.
黏附于人类宿主组织或非生物医学装置是光滑念珠菌引发感染过程中的关键步骤。这种致病性酵母的基因组编码大量黏附素，但对参考菌株进行的蛋白质组学研究表明，细胞壁中仅整合了少数几种黏附素。通过使用质谱对高黏附性临床分离株和参考菌株CBS138的细胞壁蛋白质组进行分析，我们描述了光滑念珠菌的细胞壁蛋白质组，并验证了高黏附性分离株展示出不同的黏附素整合情况这一假设。我们选择了两株临床菌株（PEU382和PEU427），它们对聚苯乙烯均具有高黏附性且表现出高表面疏水性。在生物膜形成条件下进行的细胞壁蛋白质组分析确定了所有光滑念珠菌菌株中约20种蛋白质的核心蛋白质组。此外，在高黏附性菌株中鉴定出12种黏附素样细胞壁蛋白，包括6种新型黏附素（Awp8 - 13），其中只有Awp12在CBS138中也存在。我们得出结论，这两株光滑念珠菌临床分离株的高黏附能力与细胞壁黏附素整合的增加和差异有关。未来的研究应阐明所鉴定蛋白质在光滑念珠菌感染形成过程中的作用。
