Albacete Regional Center for Biomedical Research, Castilla - La Mancha Science & Technology Park, University of Castilla-La Mancha, Albacete, Spain.
Institute for Medical Microbiology, University Medical Center Göttingen, Göttingen, Germany.
Front Cell Infect Microbiol. 2021 Nov 15;11:790465. doi: 10.3389/fcimb.2021.790465. eCollection 2021.
is among the most prevalent causes of candidiasis. Unlike , it is not capable of changing morphology between yeast and hyphal forms but instead has developed other virulence factors. An important feature is its unprecedented large repertoire of predicted cell wall adhesins, which are thought to enable adherence to a variety of surfaces under different conditions. Here, we analyzed the wall proteome of PEU1221, a high biofilm-forming clinical strain isolated from an infected central venous catheter, under biofilm-forming conditions. This isolate shows increased incorporation of putative adhesins, including eight proteins that were not detected in walls of reference strain ATCC 2001, and of which Epa22, Awp14, and Awp2e were identified for the first time. The proteomics data suggest that cluster III adhesin Awp14 is relatively abundant in PEU1221. Phenotypic studies with deletion mutants showed that Awp14 is not responsible for the high biofilm formation of PEU1221 onto polystyrene. However, mutant cells in PEU1221 background showed a slightly diminished binding to chitin and seemed to sediment slightly slower than the parental strain suggesting implication in fungal cell-cell interactions. By structural modeling, we further demonstrate similarity between the ligand-binding domains of cluster III adhesin Awp14 and those of cluster V and VI adhesins. In conclusion, our work confirms the increased incorporation of putative adhesins, such as Awp14, in high biofilm-forming isolates, and contributes to decipher the precise role of these proteins in the establishment of infections.
是假丝酵母菌病最常见的病因之一。与假丝酵母菌不同，它不能在酵母和菌丝形式之间改变形态，而是开发了其他毒力因子。一个重要特征是其前所未有的大量预测细胞壁黏附素，这些黏附素被认为能够在不同条件下黏附到各种表面。在这里，我们分析了从感染的中心静脉导管中分离出来的高生物膜形成临床分离株 PEU1221 在生物膜形成条件下的细胞壁蛋白质组。该分离株显示出增加了假定黏附素的掺入，包括在参考菌株 ATCC 2001 细胞壁中未检测到的 8 种蛋白质，其中 Epa22、Awp14 和 Awp2e 是首次被鉴定。蛋白质组学数据表明，簇 III 黏附素 Awp14 在 PEU1221 中相对丰富。用 删除突变体进行的表型研究表明，Awp14 不是导致 PEU1221 对聚苯乙烯形成高生物膜的原因。然而，在 PEU1221 背景下的 突变细胞对几丁质的结合能力略有降低，并且似乎比亲本菌株沉降略慢，表明其在真菌细胞-细胞相互作用中起作用。通过结构建模，我们进一步证明了簇 III 黏附素 Awp14 的配体结合结构域与簇 V 和 VI 黏附素的相似性。总之，我们的工作证实了高生物膜形成分离株中增加了假定黏附素的掺入，如 Awp14，并有助于解析这些蛋白质在建立 感染中的精确作用。
