Li Xiaolan, He Libang, Krom Bastiaan P, Cheng Lei, de Soet Johannes J, Deng Dong M
Guanghua School of Stomatology, Guangdong Province Key Laboratory of Stomatology, Department of Operative Dentistry and Endodontics, Sun Yat-sen University, Guangzhou 510055, China.
Academic Centre for Dentistry Amsterdam (ACTA), Department of Preventive Dentistry, Universiteit van Amsterdam and Vrije Universiteit, 1081 LA Amsterdam, The Netherlands.
Pathogens. 2022 Mar 23;11(4):387. doi: 10.3390/pathogens11040387.
is a prevalent fungal pathogen in humans, which is able to adhere to host cells and abiotic surfaces. Nicotinic acid (NA) limitation has been shown to promote the adherence of to human epithelial cells. Clinically, the elderly and hospitalized patients who are prone to -related denture stomatitis often suffer from vitamin deficiency. This study aimed to investigate adhesion to abiotic surfaces, including acrylic resin (a denture material) surfaces, cell surface hydrophobicity and adhesion gene expression. CBS138 was grown in media containing decreasing NA concentrations (40, 0.4, 0.04 and 0.004 µM). Adherence of to glass coverslips and acrylic resin was analyzed. adhesion to both surfaces generally increased with decreasing NA concentrations. The highest adhesion was found for the cells grown with 0.004 µM NA. The cell surface hydrophobicity test indicated that NA limitation enhanced hydrophobicity of cells. Quantitative PCR showed that of all adhesion genes tested, , and were significantly up-regulated in both 0.004 µM NA and 0.04 µM NA groups compared to those in the 40 µM NA group. No significant up- or down-regulation under NA limitation was observed for the other tested adhesion genes, namely , , and . NA limitation resulted in increased expression of some adhesion genes, higher surface hydrophobicity of and enhanced adhesion to abiotic surfaces. NA deficiency is likely a risk factor for -related denture stomatitis in the elderly.
是一种在人类中普遍存在的真菌病原体，能够粘附于宿主细胞和非生物表面。已表明烟酸（NA）限制会促进其对人上皮细胞的粘附。临床上，易患与相关的义齿性口炎的老年人和住院患者常患有维生素缺乏症。本研究旨在调查其对非生物表面的粘附，包括丙烯酸树脂（一种义齿材料）表面、细胞表面疏水性和粘附基因表达。CBS138在含有逐渐降低的NA浓度（40、0.4、0.04和0.004μM）的培养基中生长。分析了其对玻璃盖玻片和丙烯酸树脂的粘附情况。其对两种表面的粘附通常随着NA浓度的降低而增加。在0.004μM NA培养的细胞中发现最高的粘附力。细胞表面疏水性测试表明，NA限制增强了细胞的疏水性。定量PCR显示，在所有测试的粘附基因中，与40μM NA组相比，在0.004μM NA和0.04μM NA组中，、和均显著上调。对于其他测试的粘附基因，即、、和，在NA限制下未观察到显著的上调或下调。NA限制导致一些粘附基因表达增加、细胞表面疏水性增强以及对非生物表面的粘附增强。NA缺乏可能是老年人患与相关的义齿性口炎的一个风险因素。
