UFZ - Helmholtz Centre for Environmental Research, Permoserstraße 15, 04318 Leipzig, Germany.
UFZ - Helmholtz Centre for Environmental Research, Permoserstraße 15, 04318 Leipzig, Germany.
Biosens Bioelectron. 2016 Mar 15;77:1039-47. doi: 10.1016/j.bios.2015.10.069. Epub 2015 Oct 28.
Quinolones are antibiotics that are accredited in human and veterinary medicine but are regularly used in high quantities also in industrial livestock farming. Since these compounds are often only incompletely metabolized, significant amounts contaminate the aquatic environment and negatively impact on a variety of different ecosystems. Although there is increasing awareness of problems caused by pharmaceutical pollution, available methods for the detection and elimination of numerous pharmaceutical residues are currently inefficient or expensive. While this also applies to antibiotics that may lead to multi-drug resistance in pathogenic bacteria, aptamer-based technologies potentially offer alternative approaches for sensitive and efficient monitoring of pharmaceutical micropollutants. Using the Capture-SELEX procedure, we here describe the selection of an aptamer pool with enhanced binding qualities for fluoroquinolones, a widely used group of antibiotics in both human and veterinary medicine. The selected aptamers were shown to detect various quinolones with high specificity, while specific binding activities to structurally unrelated drugs were not detectable. The quinolone-specific aptamers bound to ofloxacin, one of the most frequently prescribed fluoroquinolone, with high affinity (KD=0.1-56.9 nM). The functionality of quinolone-specific aptamers in real water samples was demonstrated in local tap water and in effluents of sewage plants. Together, our data suggest that these aptamers may be applicable as molecular receptors in biosensors or as catcher molecules in filter systems for improved monitoring and treatment of polluted water.
喹诺酮类抗生素在人类和兽医医学中得到认可，但在工业畜牧业中也经常大量使用。由于这些化合物通常不能完全代谢，大量的污染物会污染水生环境，并对各种不同的生态系统产生负面影响。尽管人们越来越意识到药物污染带来的问题，但目前用于检测和消除大量药物残留的方法效率低下或成本高昂。虽然这也适用于可能导致病原菌产生多药耐药性的抗生素，但基于适配体的技术可能为敏感、高效地监测药物微污染物提供替代方法。在这里，我们使用Capture-SELEX 程序，描述了对氟喹诺酮类抗生素（一种在人类和兽医医学中广泛使用的抗生素）具有增强结合特性的适配体库的选择。所选择的适配体表现出对各种喹诺酮类药物具有高特异性，而对结构上不相关的药物则没有特异性结合活性。喹诺酮类特异性适配体与氧氟沙星（氟喹诺酮类中最常开处方的药物之一）具有高亲和力（KD=0.1-56.9 nM）。喹诺酮类特异性适配体在当地自来水和污水处理厂的废水中的实际水样中的功能得到了证明。总的来说，我们的数据表明，这些适配体可用作生物传感器中的分子受体，或用作过滤系统中的捕获分子，以改善受污染水的监测和处理。
