Burak Selek Mehmet, Baylan Orhan, Kutlu Ali, Özyurt Mustafa
Departments of Microbiology, Gülhane Military Medical Academy, Haydarpaşa Training Hospital, Istanbul, Turkey.
Departments of Allergy and Immunology, Gülhane Military Medical Academy, Haydarpaşa Training Hospital, Istanbul, Turkey.
Iran J Allergy Asthma Immunol. 2015 Aug;14(4):450-6.
We aimed to investigate IgG antibody levels specific to Toxocara canis (T. canis), a parasite which subsists in dog's intestine, on serum samples obtained from patients with chronic urticaria (CU) to evaluate effective risk in CU etiopathogenesis. In this study, 73 patients diagnosed with CU and 109 healthy individuals as control group, were included. Various factors such as sex, age, education and income, daily hand washing habits, history of dog owning and soil eating were questioned in patient anamnesis. T. canis IgG antibodies were detected using an enzyme linked immunosorbent assay (ELISA) kit prepared with T. canis larval excretory-secretory antigens. Positive results were confirmed with western blot (WB) WB test. We found T. canis IgG positivity in 17.8% (n=13) of patients (n=73) with CU. But we did not observe any T. canis IgG positivity in healthy controls (n=109). Low molecular weight bands (24-35 kDa) were observed in 11 samples in WB analyses while two of the samples were weakly positive. It is revealed that dog owning history increases T. canis seropositivity 12.9 times while insufficient daily hand washing habit (less than six times a day) increases seropositivity 20.7 times. Our study showed that T. canis may trigger CU since we found 17.8% seropositivity in 73 patients with CU and none in 109 healthy individuals. Moreover, various socio-demographic characteristics have been shown to affect T. canis seropositivity in patients with CU.
我们旨在调查从慢性荨麻疹（CU）患者血清样本中获取的针对犬弓首蛔虫（一种寄生于狗肠道的寄生虫）的IgG抗体水平，以评估其在CU发病机制中的有效风险。在本研究中，纳入了73名被诊断为CU的患者和109名健康个体作为对照组。在患者病史询问中，对性别、年龄、教育程度和收入、日常洗手习惯、养狗史和食土史等各种因素进行了询问。使用用犬弓首蛔虫幼虫排泄分泌抗原制备的酶联免疫吸附测定（ELISA）试剂盒检测犬弓首蛔虫IgG抗体。阳性结果用蛋白质印迹法（WB）进行确认。我们发现73名CU患者中有17.8%（n = 13）的患者犬弓首蛔虫IgG呈阳性。但在109名健康对照中未观察到任何犬弓首蛔虫IgG阳性。在WB分析中，11个样本中观察到低分子量条带（24 - 35 kDa），其中两个样本弱阳性。结果显示，养狗史使犬弓首蛔虫血清阳性率增加12.9倍，而日常洗手习惯不足（每天少于6次）使血清阳性率增加20.7倍。我们的研究表明，犬弓首蛔虫可能引发CU，因为我们在73名CU患者中发现17.8%的血清阳性，而在109名健康个体中未发现。此外，各种社会人口学特征已被证明会影响CU患者的犬弓首蛔虫血清阳性率。
