Shao Jihai, He Yaxian, Li Fan, Zhang Huiling, Chen Anwei, Luo Si, Gu Ji-Dong
College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
Hunan Provincial Key Laboratory of Farmland Pollution Control and Agricultural Resources Use, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
Ecotoxicology. 2016 Jan;25(1):225-33. doi: 10.1007/s10646-015-1582-x. Epub 2015 Nov 7.
Oleamide, a fatty acid derivative, shows inhibitory effect against the bloom-forming cyanobacterium Microcystis aeruginosa. The EC50 of oleamide on the growth of M. aeruginosa NIES-843 was 8.60 ± 1.20 mg/L. In order to elucidate the possible mechanism of toxicity of oleamide against M. aeruginosa, chlorophyll fluorescence transient, cellular ultrastructure, fatty acids composition and the transcription of the mcyB gene involved in microcystins synthesis were studied. The results of chlorophyll fluorescence transient showed that oleamide could destruct the electron accepting side of the photosystem II of M. aeruginosa NIES-843. Cellular ultrastructure examination indicated that the destruction of fatty acid constituents, the distortion of thylakoid membrane and the loss of integrity of cell membrane were associated with oleamide treatment and concentration. The damage of cellular membrane increased the release of microcystins from intact cells into the medium. Results presented in this study provide new information on the possible mechanisms involved and potential utilization of oleamide as an algicide in cyanobacterial bloom control.
油酸酰胺是一种脂肪酸衍生物，对形成水华的蓝藻铜绿微囊藻具有抑制作用。油酸酰胺对铜绿微囊藻NIES - 843生长的半数有效浓度（EC50）为8.60±1.20毫克/升。为了阐明油酸酰胺对铜绿微囊藻的可能毒性机制，研究了叶绿素荧光瞬变、细胞超微结构、脂肪酸组成以及参与微囊藻毒素合成的mcyB基因的转录情况。叶绿素荧光瞬变结果表明，油酸酰胺可破坏铜绿微囊藻NIES - 843光系统II的电子受体侧。细胞超微结构检查表明，脂肪酸成分的破坏、类囊体膜的扭曲以及细胞膜完整性的丧失与油酸酰胺处理及其浓度有关。细胞膜的损伤增加了微囊藻毒素从完整细胞向培养基中的释放。本研究结果为油酸酰胺作为蓝藻水华控制中的杀藻剂所涉及的可能机制和潜在用途提供了新信息。
