Shao Jihai, Wu Zhongxing, Yu Gongliang, Peng Xin, Li Renhui
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China.
Chemosphere. 2009 May;75(7):924-8. doi: 10.1016/j.chemosphere.2009.01.021. Epub 2009 Feb 8.
Pyrogallol is a potent allelochemical on Microcystis aeruginosa, but its allelopathic mechanism is not fully known. In order to explore this mechanism, gene expressions for prx, mcyB, psbA, recA, grpE, fabZ under pyrogallol stress were studied, and activities of the main antioxidant enzymes were also measured. The results showed that expression of grpE and recA showed no significant change under pyrogallol stress, while psbA and mcyB were up-regulated at 4 mg L(-1). Both prx and fabZ were up-regulated even under exposure to 1 mg L(-1) pyrogallol concentration. The activities of superoxide dismutase (SOD) and catalase (CAT) were enhanced under pyrogallol stress. Levels of malodialdehyde (MDA) at 2 and 4 mg L(-1) pyrogallol were significantly higher than those of the controls. It was concluded that oxidant damage is an important mechanism for the allelopathic effect of pyrogallol on M. aeruginosa.
连苯三酚是一种对铜绿微囊藻有效的化感物质，但其化感作用机制尚不完全清楚。为了探究该机制，研究了连苯三酚胁迫下prx、mcyB、psbA、recA、grpE、fabZ的基因表达情况，并测定了主要抗氧化酶的活性。结果表明，在连苯三酚胁迫下，grpE和recA的表达无显著变化，而在4 mg L(-1)时psbA和mcyB上调。即使在暴露于1 mg L(-1)连苯三酚浓度下，prx和fabZ均上调。在连苯三酚胁迫下，超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性增强。在2和4 mg L(-1)连苯三酚时，丙二醛（MDA）水平显著高于对照组。得出结论：氧化损伤是连苯三酚对铜绿微囊藻化感作用的重要机制。
