Ahn Hyo Jung, Nam Ok Hyung, Lee Hyo-Seol, Kim Eun-Cheol, Cohenca Nestor, Choi Sung Chul
Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, Korea.
Maxillofacial Tissue Regeneration and Research Center for Tooth & Periodontal Regeneration (MRC), School of Dentistry, Kyung Hee University, Seoul, Korea.
Int J Paediatr Dent. 2016 Jul;26(4):301-9. doi: 10.1111/ipd.12211. Epub 2015 Nov 7.
Immediately after the avulsed tooth is replanted, a complex inflammatory response ensues. As part of the periodontium healing process, the extracellular matrix macromolecules are essential to create the cellular environment required during healing and morphogenesis.
This study was designed to evaluate the correlation between different extra-alveolar dry times and inflammatory cytokines and matrix metalloproteinases (MMPs) as part of the periodontal ligament (PDL) gene expression.
The first phase of the study aimed testing human PDL cells ex vivo. Extracted teeth were dried for 15 and 30 min. The PDL cells were extracted and analyzed by qRT-PCR. The second phase was performed in vivo, and 36 Sprague Dawley rat first maxillary molars were extracted and replanted after 15, 30, and 60 min extra-alveolar time. We tested the levels of inflammatory cytokines and MMPS in periodontal tissue at 3, 7, and 28 days after tooth replantation. The replanted area was dissected, grounded, and analyzed by RT-PCR.
Expressions of IL-1β, IL-6, TNF-α, and MMP-3 and MMP-9 were significantly higher in the replanted teeth. Extended dry time had a direct correlation with induction of pro-inflammatory cytokine and MMPs in PDL cells.
Our study showed that pro-inflammatory cytokines were more significantly expressed in the tissues surrounding the replanted teeth. Future research must be undertaken to additionally confirm the release of these cytokines and be focused on the inhibition of these cytokines to reduce inflammation of replanted teeth.
牙齿再植后立即会发生复杂的炎症反应。作为牙周组织愈合过程的一部分，细胞外基质大分子对于创建愈合和形态发生过程中所需的细胞环境至关重要。
本研究旨在评估不同的牙槽外干燥时间与作为牙周膜（PDL）基因表达一部分的炎性细胞因子和基质金属蛋白酶（MMPs）之间的相关性。
研究的第一阶段旨在对人PDL细胞进行体外测试。将拔除的牙齿干燥15分钟和30分钟。提取PDL细胞并通过qRT-PCR进行分析。第二阶段在体内进行，拔除36颗Sprague Dawley大鼠的上颌第一磨牙，并在牙槽外放置15、30和60分钟后再植。我们在牙齿再植后3、7和28天测试了牙周组织中炎性细胞因子和MMPs的水平。对再植区域进行解剖、研磨，并通过RT-PCR进行分析。
再植牙齿中IL-1β、IL-6、TNF-α以及MMP-3和MMP-9的表达明显更高。延长干燥时间与PDL细胞中促炎性细胞因子和MMPs的诱导直接相关。
我们的研究表明，促炎性细胞因子在再植牙齿周围的组织中表达更为显著。未来必须进一步开展研究以证实这些细胞因子的释放，并专注于抑制这些细胞因子以减轻再植牙齿的炎症。
