大鼠牙齿即刻与延迟再植后牙周软组织的差异基因表达谱：一项初步研究。

Differential gene expression profiles of periodontal soft tissue from rat teeth after immediate and delayed replantation: a pilot study.

作者信息

Chae Yong Kwon, Shin Seo Young, Kang Sang Wook, Choi Sung Chul, Nam Ok Hyung

机构信息

Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, Korea.

Department of Dentistry, Graduate School, Kyung Hee University, Seoul, Korea.

出版信息

J Periodontal Implant Sci. 2022 Apr;52(2):127-140. doi: 10.5051/jpis.2104300215.

DOI:10.5051/jpis.2104300215

PMID:35505574

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9064781/

Abstract

PURPOSE

In dental avulsion, delayed replantation usually has an uncertain prognosis. After tooth replantation, complex inflammatory responses promote a return to periodontal tissue homeostasis. Various types of cytokines are produced in the inflammatory microenvironment, and these cytokines determine the periodontal tissue response. This study aimed to identify the gene expression profiles of replanted teeth and evaluate the functional differences between immediate and delayed replantation.

METHODS

Maxillary molars from Sprague-Dawley rats were extracted, exposed to a dry environment, and then replanted. The animals were divided into 2 groups according to the extra-oral time: immediate replantation (dry for 5 minutes) and delayed replantation (dry for 60 minutes). Either 3 or 7 days after replantation, the animals were sacrificed. Periodontal soft tissues were harvested for mRNA sequencing. Hallmark gene set enrichment analysis was performed to predict the function of gene-gene interactions. The normalized enrichment score (NES) was calculated to determine functional differences.

RESULTS

The hallmark gene sets enriched in delayed replantation at 3 days were oxidative phosphorylation (NES=2.82, 0.001) and tumor necrosis factor-alpha (TNF-α) signaling via the nuclear factor kappa light chain enhancer of activated B cells (NF-κB) pathway (NES=1.52, 0.034). At 7 days after delayed replantation, TNF-α signaling via the NF-κB pathway (NES=-1.82, 0.002), angiogenesis (NES=-1.66, 0.01), and the transforming growth factor-beta signaling pathway (NES=-1.46, 0.051) were negatively highlighted.

CONCLUSIONS

Differentially expressed gene profiles were significantly different between immediate and delayed replantation. TNF-α signaling via the NF-κB pathway was marked during the healing process. However, the enrichment score of this pathway changed in a time-dependent manner between immediate and delayed replantation.

摘要

目的

在牙脱位病例中，延期再植的预后通常不确定。牙齿再植后，复杂的炎症反应促使牙周组织恢复稳态。炎症微环境中会产生多种细胞因子，这些细胞因子决定了牙周组织的反应。本研究旨在确定再植牙的基因表达谱，并评估即刻再植与延期再植之间的功能差异。

方法

从Sprague-Dawley大鼠中拔除上颌磨牙，使其暴露于干燥环境中，然后进行再植。根据口腔外时间将动物分为2组：即刻再植（干燥5分钟）和延期再植（干燥60分钟）。再植后3天或7天，处死动物。收集牙周软组织进行mRNA测序。进行标志性基因集富集分析以预测基因-基因相互作用的功能。计算标准化富集分数（NES）以确定功能差异。

结果

在再植后3天，延期再植中富集的标志性基因集是氧化磷酸化（NES = 2.82，0.001）和通过活化B细胞核因子κB轻链增强子（NF-κB）途径的肿瘤坏死因子-α（TNF-α）信号传导（NES = 1.52，0.034）。在延期再植后7天，通过NF-κB途径的TNF-α信号传导（NES = -1.82，0.002）、血管生成（NES = -1.66，0.01）和转化生长因子-β信号传导途径（NES = -1.46，0.051）被负向突出显示。