Zhu Yingjie, Sun Yueli, Cai Yuchen, Sha Ou, Jiang Wenqi
State Key Laboratory of Oncology in Southern China, Collaborative Innovation Center for Cancer Medicine, Sun Yat‑sen University Cancer Center, Guangzhou, Guangdong 510060, P.R. China.
Department of Thoracic Oncology, Guangdong Lung Cancer Institute, Guangdong General Hospital and Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, P.R. China.
Mol Med Rep. 2016 Jan;13(1):403-11. doi: 10.3892/mmr.2015.4531. Epub 2015 Nov 6.
Previous studies have indicated that trichosanthin (TCS) exerts antitumor activity by inducing apoptosis in numerous tumor cell lines. However, the effects of TCS on lymphoma remain to be elucidated. The current study demonstrated that TCS inhibited the proliferation of thirteen lymphoma cell lines in a dose‑dependent manner, with SU‑DHL‑2 cells exhibiting the greatest sensitivity to TCS. Treatment of SU‑DHL‑2 cells with TCS led to cell cycle arrest at the S to G2/M phase transition. Furthermore, flow cytometric analysis, Hoechst 33258 staining and western blotting indicated that TCS induced the apoptosis of SU‑DHL‑2 cells in a time‑ and concentration‑dependent manner. In addition, the activation of caspase‑3 and ‑7 and poly (ADP‑ribose) polymerase were observed. Pharmacological pan-caspase inhibition was observed to reduce TCS‑induced apoptosis. Inhibition of caspase‑8 or ‑9 alone was observed to partially reverse the effect of TCS on apoptosis. In conclusion, the current study indicates that TCS may induce apoptosis in SU‑DHL‑2 cells via the extrinsic and intrinsic pathways.
先前的研究表明，天花粉蛋白（TCS）通过诱导多种肿瘤细胞系凋亡发挥抗肿瘤活性。然而，TCS对淋巴瘤的作用仍有待阐明。当前研究表明，TCS以剂量依赖性方式抑制13种淋巴瘤细胞系的增殖，其中SU-DHL-2细胞对TCS表现出最大敏感性。用TCS处理SU-DHL-2细胞导致细胞周期停滞在S期至G2/M期转换。此外，流式细胞术分析、Hoechst 33258染色和蛋白质印迹表明，TCS以时间和浓度依赖性方式诱导SU-DHL-2细胞凋亡。此外，观察到半胱天冬酶-3和-7以及聚（ADP-核糖）聚合酶的激活。观察到药理学上的泛半胱天冬酶抑制可减少TCS诱导的凋亡。单独抑制半胱天冬酶-8或-9可部分逆转TCS对凋亡的影响。总之，当前研究表明，TCS可能通过外源性和内源性途径诱导SU-DHL-2细胞凋亡。
