Wood K V, Lam Y A, Seliger H H, McElroy W D
Department of Chemistry, University of California, San Diego, La Jolla 92093.
Science. 1989 May 12;244(4905):700-2. doi: 10.1126/science.2655091.
Eleven complementary DNA (cDNA) clones were generated from messenger RNA isolated from abdominal light organs of the bioluminescent click beetle, Pyrophorus plagiophthalamus. When expressed in Escherichia coli, these clones can elicit bioluminescence that is readily visible. The clones code for luciferases of four types, distinguished by the colors of bioluminescence they catalyze: green (546 nanometers), yellow-green (560 nanometers), yellow (578 nanometers), and orange (593 nanometers). The amino acid sequences of the different luciferases are 95 to 99 percent identical with each other, but are only 48 percent identical with the sequence of firefly luciferase (Photinus pyralis). Because of the different colors, these clones may be useful in experiments in which multiple reporter genes are needed.
从发光叩头虫（Pyrophorus plagiophthalamus）腹部发光器官分离出的信使核糖核酸（mRNA）中产生了11个互补脱氧核糖核酸（cDNA）克隆。当这些克隆在大肠杆菌中表达时，它们能够引发清晰可见的生物发光。这些克隆编码四种类型的荧光素酶，根据它们催化的生物发光颜色区分：绿色（546纳米）、黄绿色（560纳米）、黄色（578纳米）和橙色（593纳米）。不同荧光素酶的氨基酸序列彼此之间有95%至99%的同源性，但与萤火虫荧光素酶（Photinus pyralis）的序列只有48%的同源性。由于颜色不同，这些克隆可能在需要多个报告基因的实验中有用。
