新生小鼠的一系列肠道损伤模型。
A spectrum of intestinal injury models in neonatal mice.
作者信息
Zani Augusto, Zani-Ruttenstock Elke, Peyvandi Forouhideh, Lee Carol, Li Bo, Pierro Agostino
机构信息
Division of General and Thoracic Surgery, Physiology and Experimental Medicine Program, The Hospital for Sick Children, 1526-555 University Ave, Toronto, ON, M5G 1X8, Canada.
University of Toronto, Toronto, Canada.
出版信息
Pediatr Surg Int. 2016 Jan;32(1):65-70. doi: 10.1007/s00383-015-3813-x. Epub 2015 Nov 9.
PURPOSE
To compare the degree of necrotizing enterocolitis (NEC)-like damage under different stress conditions in neonatal mice.
METHODS
5-day-old C57BL/6 mice were assigned to: (A) breastfed and no stress factors; (B) breastfed+maternal separation (3 h daily); (C) breastfed+hypoxia+lipopolysaccharide (LPS-4 mg/kg/day); (D) hyperosmolar formula+hypoxia+LPS. Mice were killed at 9 days of life. Ileum and colon were stained for hematoxylin/eosin and blindly assessed. A scoring ≥2 was considered NEC. Data were compared using one-way ANOVA and reported as median (range).
RESULTS
Ileum-Mucosal injury was mild in group B (0.0-1). Hypoxia+LPS induced greater injury in group C (1.6, 1-2.5; p < 0.0001 to B) and D (2, 0.5-3.5; p < 0.0001 to B). There were no differences between group C and D (p = n.s.). There were no cases of NEC in group A or B, whereas NEC was present in 36 % group C and 68 % group D mice. Colon-a similar degree of mucosal injury was observed among group B (2, 1-3), C (1.7, 0-3) and D (1.5, 1-3; p = n.s.). NEC was present in 75 % of group B, 50 % of group C and 86 % of group D.
CONCLUSION
These models establish a spectrum of intestinal injury and are useful to investigate the variability of neonatal intestinal diseases, such as NEC.
目的
比较新生小鼠在不同应激条件下坏死性小肠结肠炎（NEC）样损伤的程度。
方法
将5日龄的C57BL/6小鼠分为：（A）母乳喂养且无应激因素组；（B）母乳喂养+母婴分离（每天3小时）组；（C）母乳喂养+缺氧+脂多糖（LPS-4mg/kg/天）组；（D）高渗配方奶+缺氧+LPS组。在小鼠9日龄时处死。对回肠和结肠进行苏木精/伊红染色并进行盲法评估。评分≥2被认为是NEC。数据采用单因素方差分析进行比较，并以中位数（范围）报告。
结果
回肠——B组黏膜损伤较轻（0.0-1）。缺氧+LPS在C组（1.6，1-2.5；与B组相比p<0.0001）和D组（2，0.5-3.5；与B组相比p<0.0001）诱导了更严重的损伤。C组和D组之间无差异（p=无统计学意义）。A组或B组无NEC病例，而C组36%的小鼠和D组68%的小鼠存在NEC。结肠——B组（2，1-3）、C组（1.7，0-3）和D组（1.5，1-3；p=无统计学意义）观察到相似程度的黏膜损伤。B组75%、C组50%和D组86%存在NEC。
结论
这些模型建立了一系列肠道损伤，有助于研究新生儿肠道疾病如NEC的变异性。
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