Kononikhin A S, Tiys E S, Nosovsky A M, Dobrokhotov I V, Ivanisenko V A, Nikolaev E N, Novoselova N M, Custaud M A, Larina I M
Aviakosm Ekolog Med. 2015;49(4):15-9.
The study was aimed at tracking the proteomic profile of urine in 8 normal volunteers to 5-day dry immersion (DI). The proteome composition was determined by chromatography-mass spectrometry on high-efficient on-line liquid nano chromatograph Agilent 1100; complementary information about the protein spectra was obtained by dint of mass-spectrometer MaXis Impact 4G and hybrid mass-spectrometer LTQ-FT. Functional associations between proteins and biological functions were analyzed using computer system ANDCell (Associative Networks Discovery in Cells). A total of 256 proteins were identified; for 43 proteins difference in the detection rate during the baseline data collection and on DI day 4 exceeded 20%.
该研究旨在追踪8名正常志愿者在5天干浸（DI）期间尿液的蛋白质组概况。蛋白质组组成通过高效在线液相纳米色谱仪安捷伦1100上的色谱-质谱法测定；借助质谱仪MaXis Impact 4G和混合质谱仪LTQ-FT获得有关蛋白质谱的补充信息。使用计算机系统ANDCell（细胞中的关联网络发现）分析蛋白质与生物学功能之间的功能关联。总共鉴定出256种蛋白质；43种蛋白质在基线数据收集期间和DI第4天的检测率差异超过20%。
