Pastukhova L Kh, Kononikhin A S, Tiĭs E S, Popova I A, Dobrokhotov I V, Ivanisenko V A, Nikolaev E N, Larina I M
Ross Fiziol Zh Im I M Sechenova. 2013 Aug;99(8):945-59.
In order to find markers to assess the functional state of the cardiovascular system before and after spaceflight (first and seventh day after landing), we analyzed the urine proteome in ten cosmonauts aged of 35 to 51 years who have completed 169 to 199-day spaceflight onboard the ISS. A special sample preparation was performed, followed by liquid chromatography-mass spectrometry. Liquid chromatography-mass spectrometry of the minor proteins was performed on a nano-HPLC Agilent 1100 system (Agilent Technologies Inc., USA) in combination with a LTQ-FT Ultra mass spectrometer (Thermo, Germany). A total of 238 proteins was identified. According to the TIGER database, a tissue origin was established for 129 proteins. We identified 20 proteins related to the cardiovascular system. It was found that changes in cosmonauts' urine proteome comprehensively reflect the adaptive responses of cardiovascular, renal and neuroendocrine systems to long-term microgravity conditions.
为了找到用于评估航天飞行前后（着陆后第一天和第七天）心血管系统功能状态的标志物，我们分析了10名年龄在35至51岁之间、在国际空间站上完成了169至199天航天飞行的宇航员的尿液蛋白质组。进行了特殊的样品制备，随后进行液相色谱 - 质谱分析。微量蛋白质的液相色谱 - 质谱分析在配备LTQ - FT Ultra质谱仪（德国赛默飞世尔科技公司）的纳升液相色谱安捷伦1100系统（美国安捷伦科技公司）上进行。共鉴定出238种蛋白质。根据TIGER数据库，确定了129种蛋白质的组织来源。我们鉴定出20种与心血管系统相关的蛋白质。结果发现，宇航员尿液蛋白质组的变化全面反映了心血管、肾脏和神经内分泌系统对长期微重力条件的适应性反应。
