长期轨道飞行后人尿蛋白质谱的变化

Changes of protein profile of human urine after long-term orbital flights.

作者信息

Pastushkova L Kh, Valeeva O A, Kononikhin A S, Nikolaev E N, Larina I M, Dobrokhotov I V, Popov I A, Pochuev V I, Kireev K S

机构信息

State Scientific Center of the Russian Federation Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia; N. M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow; Yu. A. Gagarin Research and Test Cosmonaut Training Center, Zvezdnyi Gorodok, Moscow Region; Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.

出版信息

Bull Exp Biol Med. 2013 Dec;156(2):201-4. doi: 10.1007/s10517-013-2310-2.

DOI:10.1007/s10517-013-2310-2

PMID:24319748

Abstract

We analyzed protein profile of urine samples obtained from 7 Russian cosmonauts (age 35-51 years), participants of space flights on the International Space Station lasting for 169-199 days. Gradient chromatography with linear increase of eluent proportion was carried out in a system consisting of an Agilent 1100 chromatograph (Agilent Technologies Inc.) and a hybrid mass-spectrometer LTQ-FT Ultra (Thermo). The obtained results help to understand changes in the human body induced by space flight factors.

摘要

我们分析了7名俄罗斯宇航员（年龄在35至51岁之间）的尿液样本蛋白质谱，这些宇航员参与了在国际空间站持续169至199天的太空飞行。在由安捷伦1100色谱仪（安捷伦科技公司）和混合型质谱仪LTQ-FT Ultra（赛默飞世尔科技公司）组成的系统中，进行了洗脱液比例线性增加的梯度色谱分析。所得结果有助于了解太空飞行因素对人体造成的变化。