Kim Jangho, Park Subeom, Kim Yeon Ju, Jeon Chang Su, Lim Ki Taek, Seonwoo Hoon, Cho Sung-Pyo, Chung Taek Dong, Choung Pill-Hoon, Choung Yun-Hoon, Hong Byung Hee, Chung Jong Hoon
J Biomed Nanotechnol. 2015 Nov;11(11):2024-33. doi: 10.1166/jbn.2015.2137.
The development of an efficient platform for the growth and neuronal differentiation of stem cells is crucial for autologous cell therapy and tissue engineering to treat various neuronal disorders and neurodegenerative diseases. In this study, we describe the use of highly uniform graphene platforms that provide unique environments where unusual three-dimensional spheroids of human mesenchymal stem cells (hMSCs) are formed, which is advantageous for the differentiation of hMSCs into neurons. We suppose that graphene regulates the interactions at cell-substrate or cell-cell interfaces, consequently promoting the neurogenesis of hMSCs as well as the outgrowth of neurites, which was evidenced by the graphene-induced upregulation of early neurogenesis-related genes. We also demonstrated that the differentiated neurons from hMSCs on graphene are notably sensitive to external ion stimulation, and their neuronal properties can be maintained even after detaching and re-seeding onto a normal cell culture substrate, suggesting the enhanced maturity of resulting neuronal cells. Thus, we conclude that monolayer graphene is capable of regulating the growth and neural differentiation of hMSCs, which would provide new insight and strategy not only for autologous stem cell therapy but for tissue engineering and regenerative medicine based on graphene scaffolds.
开发一个用于干细胞生长和神经元分化的高效平台，对于自体细胞治疗和组织工程来治疗各种神经元疾病和神经退行性疾病至关重要。在本研究中，我们描述了使用高度均匀的石墨烯平台，该平台提供了独特的环境，在其中形成了人骨髓间充质干细胞（hMSCs）异常的三维球体，这有利于hMSCs向神经元分化。我们推测石墨烯调节细胞-基质或细胞-细胞界面的相互作用，从而促进hMSCs的神经发生以及神经突的生长，这通过石墨烯诱导的早期神经发生相关基因的上调得到证明。我们还证明，在石墨烯上由hMSCs分化而来的神经元对外部离子刺激特别敏感，并且即使在分离并重新接种到正常细胞培养基质上后，它们的神经元特性仍能保持，这表明所得神经元细胞的成熟度提高。因此，我们得出结论，单层石墨烯能够调节hMSCs的生长和神经分化，这不仅将为自体干细胞治疗，而且为基于石墨烯支架的组织工程和再生医学提供新的见解和策略。
