对含有荧光探针的卵磷脂双分子层的核磁共振研究。
Nuclear magnetic resonance studies of lecithin bimolecular leaflets with incorporated fluorescent probes.
作者信息
Podo F, Blasie J K
出版信息
Proc Natl Acad Sci U S A. 1977 Mar;74(3):1032-6. doi: 10.1073/pnas.74.3.1032.
Abstract
Pulse Fourier transform proton magnetic resonance techniques have been used to determine (i) the precise location of four membrane fluorescent probe molecules in lipid bilayer model membranes above the gel-to-liquid crystal transition temperature, tm; (ii) the dynamical perturbation of the host lipid molecules induced by incorporation of the probe into the bilayer. The regions of maximal perturbation induced by these probes do not necessarily coincide with their actual location in the bilayer.
摘要
脉冲傅里叶变换质子磁共振技术已被用于确定
（i）在高于凝胶-液晶转变温度tm的脂质双层模型膜中，四个膜荧光探针分子的精确位置；（ii）由于探针掺入双层膜而引起的主体脂质分子的动态扰动。这些探针引起的最大扰动区域不一定与它们在双层膜中的实际位置一致。
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本文引用的文献
1
GROUP MEDICINE.集体医疗
Am J Public Health (N Y). 1919 May;9(5):358-62. doi: 10.2105/ajph.9.5.358.
2
The use of NMR spectra of sonicated phospholipid dispersions in studies of interactions with the bilayer.
FEBS Lett. 1971 Nov 1;18(2):331-334. doi: 10.1016/0014-5793(71)80479-9.
3
Fluorescent probes of biological membranes.生物膜的荧光探针
Proc Natl Acad Sci U S A. 1970 Oct;67(2):579-89. doi: 10.1073/pnas.67.2.579.
4
Mechanism of sonication of aqueous egg yolk lecithin dispersions and nature of the resultant particles.水性蛋黄卵磷脂分散体的超声处理机制及所得颗粒的性质。
Biochim Biophys Acta. 1972 Jan 27;260(1):49-58. doi: 10.1016/0005-2760(72)90073-2.
5
Temperature and frequency dependence of longitudinal proton relaxation times in sonicated lecithin dispersions.超声处理的卵磷脂分散液中纵向质子弛豫时间的温度和频率依赖性
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6
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