Podo F, Blasie J K
Proc Natl Acad Sci U S A. 1977 Mar;74(3):1032-6. doi: 10.1073/pnas.74.3.1032.
Pulse Fourier transform proton magnetic resonance techniques have been used to determine (i) the precise location of four membrane fluorescent probe molecules in lipid bilayer model membranes above the gel-to-liquid crystal transition temperature, tm; (ii) the dynamical perturbation of the host lipid molecules induced by incorporation of the probe into the bilayer. The regions of maximal perturbation induced by these probes do not necessarily coincide with their actual location in the bilayer.
(i)在高于凝胶-液晶转变温度tm的脂质双层模型膜中,四个膜荧光探针分子的精确位置;(ii)由于探针掺入双层膜而引起的主体脂质分子的动态扰动。这些探针引起的最大扰动区域不一定与它们在双层膜中的实际位置一致。