基于混合导电聚合物/富勒烯纳米颗粒光敏剂的光动力疗法
Photodynamic Therapy with Blended Conducting Polymer/Fullerene Nanoparticle Photosensitizers.
作者信息
Doshi Mona, Gesquiere Andre J
机构信息
NanoScience Technology Center, University of Central Florida; Department of Chemistry, University of Central Florida.
NanoScience Technology Center, University of Central Florida; Department of Chemistry, University of Central Florida; Department of Materials Science and Engineering, University of Central Florida; CREOL, The College of Optics and Photonics, University of Central Florida;
出版信息
J Vis Exp. 2015 Oct 28(105):e53038. doi: 10.3791/53038.
In this article a method for the fabrication and reproducible in-vitro evaluation of conducting polymer nanoparticles blended with fullerene as the next generation photosensitizers for Photodynamic Therapy (PDT) is reported. The nanoparticles are formed by hydrophobic interaction of the semiconducting polymer MEH-PPV (poly[2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylenevinylene]) with the fullerene PCBM (phenyl-C61-butyric acid methyl ester) in the presence of a non-compatible solvent. MEH-PPV has a high extinction coefficient that leads to high rates of triplet formation, and efficient charge and energy transfer to the fullerene PCBM. The latter processes enhance the efficiency of the PDT system through fullerene assisted triplet and radical formation, and ultrafast deactivation of MEH-PPV excited stated. The results reported here show that this nanoparticle PDT sensitizing system is highly effective and shows unexpected specificity to cancer cell lines.
本文报道了一种制备和可重复体外评估与富勒烯混合的导电聚合物纳米颗粒的方法，该纳米颗粒作为光动力疗法（PDT）的下一代光敏剂。纳米颗粒是在不相容溶剂存在下，通过半导体聚合物MEH-PPV（聚[2-甲氧基-5-(2-乙基己氧基)-1,4-亚苯基亚乙烯基]）与富勒烯PCBM（苯基-C61-丁酸甲酯）的疏水相互作用形成的。MEH-PPV具有高消光系数，导致三线态形成速率高，并能有效地将电荷和能量转移到富勒烯PCBM。后一过程通过富勒烯辅助三线态和自由基形成以及MEH-PPV激发态的超快失活提高了PDT系统的效率。此处报道的结果表明，这种纳米颗粒PDT敏化系统非常有效，并且对癌细胞系表现出意想不到的特异性。
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