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大黄游离蒽醌提取物对高糖培养系膜细胞增殖及细胞外基质积聚的影响。

Effects of Free Anthraquinones Extract from the Rhubarb on Cell Proliferation and Accumulation of Extracellular Matrix in High Glucose Cultured-Mesangial Cells.

机构信息

Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou 221004, Jiangsu, China.

Department of Urology, Xuzhou Central Hospital, The Affiliated School of Clinical Medicine of Xuzhou Medical College, Xuzhou 221009, Jiangsu, China.

出版信息

Korean J Physiol Pharmacol. 2015 Nov;19(6):485-9. doi: 10.4196/kjpp.2015.19.6.485. Epub 2015 Oct 16.

DOI:10.4196/kjpp.2015.19.6.485
PMID:26557014
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4637350/
Abstract

Diabetic nephropathy (DN) is the leading cause of end-stage failure of the kidney, but the efficacy of currently available strategies for the prevention of DN remains unsatisfactory. In this study, we investigated the effects of free anthraquinones (FARs) extract, which was extracted from the rhubarb and purified by macroporous resin DM130 with gradient mixtures of ethanol/water as the lelution solvents, in high glucose-cultured glomerular mesangial cells (MCs). The cell proliferation was determined by CCK-8 assay, the levels of TGF-β1, CTGF, ColIV and FN proteins in the supernatant of MCs were measured by ELISA assays, and the mRNA levels of these four genes were detected by RT-PCR. The results showed that the increased proliferation of MCs, the mRNA levels and protein expression of TGF-β1, CTGF, ColIV and FN induced by high glucose were inhibited after the treatment with the FARs extract. This indicated that FARs extract could inhibit cell proliferation and the expression of main extracellular matrix induced by high glucose in MCs. The FARs extract exhibited potential values for prophylaxis and therapy of DN.

摘要

糖尿病肾病（DN）是导致肾脏终末期衰竭的主要原因，但目前用于预防 DN 的策略的疗效仍不尽如人意。在这项研究中，我们研究了大黄中提取的游离蒽醌（FARs）提取物的作用，该提取物通过大孔树脂 DM130 用乙醇/水的梯度混合物作为洗脱溶剂进行纯化，在高糖培养的肾小球系膜细胞（MCs）中。通过 CCK-8 测定法测定细胞增殖，通过 ELISA 测定法测定 MCs 上清液中 TGF-β1、CTGF、ColIV 和 FN 蛋白的水平，通过 RT-PCR 检测这四个基因的 mRNA 水平。结果表明，FARs 提取物可抑制高糖诱导的 MCs 增殖、TGF-β1、CTGF、ColIV 和 FN 的 mRNA 水平和蛋白表达增加。这表明 FARs 提取物可抑制高糖诱导的 MCs 中的细胞增殖和主要细胞外基质的表达。FARs 提取物对 DN 的预防和治疗具有潜在价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/933f/4637350/e40b50309a3d/kjpp-19-485-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/933f/4637350/c6f677e8635a/kjpp-19-485-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/933f/4637350/dabd88e7e146/kjpp-19-485-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/933f/4637350/1bfd7b9ab895/kjpp-19-485-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/933f/4637350/e40b50309a3d/kjpp-19-485-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/933f/4637350/c6f677e8635a/kjpp-19-485-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/933f/4637350/dabd88e7e146/kjpp-19-485-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/933f/4637350/1bfd7b9ab895/kjpp-19-485-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/933f/4637350/e40b50309a3d/kjpp-19-485-g004.jpg

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