Chen Xiao, Wang Dong-Dong, Wei Tong, He Su-Mei, Zhang Guan-Ying, Wei Qun-Li
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, Jiangsu 221004, P.R. China; Department of Pharmacy, The People's Hospital of Jiangyin, Jiangyin, Jiangsu 214400, P.R. China.
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, Jiangsu 221004, P.R. China.
Exp Ther Med. 2016 Jun;11(6):2561-2566. doi: 10.3892/etm.2016.3194. Epub 2016 Mar 24.
Diabetic nephropathy (DN) exhibits a deteriorating course that may lead to end-stage renal failure. Astragalosides have been clinically tested for the treatment of DN, but the mechanism is unclear at present. In this study, the effects of astragalosides were investigated on high glucose-induced proliferation and expression of transforming growth factor-β1 (TGF-β1), connective tissue growth factor (CTGF), type IV collagen (colIV) and fibronectin (FN) in glomerular mesangial cells (MCs). Cell proliferation was determined by 5-bromo-2'-deoxyuridine assay, and the expression of TGF-β1, CTGF, colIV and FN mRNA and proteins in MCs was detected by reverse transcription-polymerase chain reaction and ELISA assay, respectively. The results showed that high glucose clearly induced the proliferation of MCs and increased the expression of TGF-β1, CTGF, colIV and FN. Treatment with 50, 100, 200 µg/ml astragalosides inhibited cell proliferation and the expression of TGF-β1, CTGF, colIV and FN induced by high glucose. Thus, it is concluded that astragalosides inhibit the increased cell proliferation and expression of major extracellular matrix proteins that are induced by high glucose, indicating their value for the prophylaxis and therapy of DN.
糖尿病肾病（DN）呈现出病情不断恶化的过程，可能导致终末期肾衰竭。黄芪甲苷已针对DN的治疗进行了临床试验，但目前其机制尚不清楚。在本研究中，研究了黄芪甲苷对高糖诱导的肾小球系膜细胞（MCs）增殖以及转化生长因子-β1（TGF-β1）、结缔组织生长因子（CTGF）、IV型胶原（colIV）和纤连蛋白（FN）表达的影响。通过5-溴-2'-脱氧尿苷检测法测定细胞增殖，并分别通过逆转录-聚合酶链反应和ELISA检测法检测MCs中TGF-β1、CTGF、colIV和FN的mRNA及蛋白表达。结果显示，高糖明显诱导了MCs的增殖，并增加了TGF-β1、CTGF、colIV和FN的表达。用50、100、200μg/ml黄芪甲苷处理可抑制高糖诱导的细胞增殖以及TGF-β1、CTGF、colIV和FN的表达。因此，得出结论，黄芪甲苷可抑制高糖诱导的细胞增殖增加以及主要细胞外基质蛋白的表达，表明其在DN预防和治疗中的价值。
