Schoenenberger G A, Monnier M
Proc Natl Acad Sci U S A. 1977 Mar;74(3):1282-6. doi: 10.1073/pnas.74.3.1282.
A peptide that induces slow-wave (delta) and spindles electroencephalogram enhancement after intraventricular (brain) infusion has been isolated from rabbits and given the name delta-sleep-inducing peptide (DSIP). Amino acid seqeunce: Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu. This compound, five possible metabolic products (containing residues 1--8, 2--9, 2--8, 1--4, and 5--9), two nonapeptide analogues with two amino acids exchanged, and a related tripeptide (Trp-Ser-Glu) were synthesized. All nine synthetic peptides were infused intraventricularly in rabbits under double-blind conditions. A total of 58 rabbits including controls were evaluated. The electroencephalogram leads from the neocortex and the archicortex were directly fast-Fourier transformed and analyzed by a Univac 1108 computer system. Only the delta-sleep-inducing peptide (snythetic) showed significant and specific enhancement/induction of delta and spindle electroencephalogram patterns.
一种在脑室内（脑）注入后能诱导慢波（δ）和纺锤波脑电图增强的肽已从兔子体内分离出来，并被命名为δ-睡眠诱导肽（DSIP）。氨基酸序列：色氨酸-丙氨酸-甘氨酸-甘氨酸-天冬氨酸-丙氨酸-丝氨酸-甘氨酸-谷氨酸。合成了该化合物、五种可能的代谢产物（含有第1 - 8、2 - 9、2 - 8、1 - 4和5 - 9位残基）、两种有两个氨基酸交换的九肽类似物以及一种相关的三肽（色氨酸-丝氨酸-谷氨酸）。所有九种合成肽在双盲条件下经脑室内注入兔子体内。总共对包括对照组在内的58只兔子进行了评估。来自新皮层和古皮层的脑电图导联直接进行快速傅里叶变换，并由一台Univac 1108计算机系统进行分析。只有δ-睡眠诱导肽（合成的）显示出对δ和纺锤波脑电图模式有显著且特异性的增强/诱导作用。
