Iyer K S, Marks G A, Kastin A J, McCann S M
Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235.
Proc Natl Acad Sci U S A. 1988 May;85(10):3653-6. doi: 10.1073/pnas.85.10.3653.
To examine the role of delta sleep-inducing peptide (DSIP) in sleep-related growth hormone (GH) release, male rats were deprived of sleep for 4 hr by placing them on a slowly rotating wheel. Sleep deprivation by this method caused a significant increase in GH release, as indicated by the increase in plasma GH concentrations (P less than 0.01), and also in the amount of slow-wave sleep (SWS) (P less than 0.001) above initial values after removal of the animals from the rotating wheel. These increases were blocked by microinjection into the third cerebral ventricle of highly specific antiserum to DSIP. In control rats receiving an equal volume of normal rabbit serum, the significant increase in plasma GH as well as SWS remained after removal of the rats from the wheel. The increased release of endogenous DSIP in the sleep-deprived animals may have caused an increase in SWS as well as plasma GH. Since DSIP increases plasma GH after its injection into the third cerebral ventricle and since passive immunization against DSIP blocks the increase in SWS and GH release that follows the 4 hr of sleep deprivation, the results suggest that DSIP can be a physiological stimulus for sleep-related GH release as well as for the induction of SWS.
为了研究δ睡眠诱导肽（DSIP）在与睡眠相关的生长激素（GH）释放中的作用，将雄性大鼠置于缓慢旋转的轮子上使其睡眠剥夺4小时。通过这种方法进行的睡眠剥夺导致GH释放显著增加，血浆GH浓度升高表明了这一点（P<0.01），并且在将动物从旋转轮上取下后，慢波睡眠（SWS）量也高于初始值（P<0.001）。这些增加被向第三脑室微量注射针对DSIP的高特异性抗血清所阻断。在接受等量正常兔血清的对照大鼠中，将大鼠从轮子上取下后，血浆GH以及SWS的显著增加仍然存在。睡眠剥夺动物体内内源性DSIP释放的增加可能导致了SWS以及血浆GH的增加。由于DSIP注入第三脑室后会增加血浆GH，并且由于针对DSIP的被动免疫阻断了4小时睡眠剥夺后SWS和GH释放的增加，结果表明DSIP可以作为与睡眠相关的GH释放以及SWS诱导的生理刺激物。
