Mugwagwa Andrew T, Gadaga Louis L, Pote William, Tagwireyi Dexter
Drug and Toxicology Information Service (DaTIS), School of Pharmacy and Department of Clinical Pharmacology, College of Health Sciences, University of Zimbabwe, P.O. Box A 178, Avondale, Harare, Zimbabwe.
Drug and Toxicology Information Service (DaTIS), School of Pharmacy and Department of Clinical Pharmacology, College of Health Sciences, University of Zimbabwe, P.O. Box A 178, Avondale, Harare, Zimbabwe ; Department of Preclinical Veterinary Studies, Faculty of Veterinary Sciences, University of Zimbabwe, P.O. Box MP, Mount Pleasant, Harare, Zimbabwe.
J Neurodegener Dis. 2015;2015:242505. doi: 10.1155/2015/242505. Epub 2015 Oct 8.
Crinum macowanii has been found to contain alkaloids that have activity against acetylcholinesterase enzyme in vitro. The present study was undertaken to investigate the in vivo ability of hydroethanolic crude extract of Crinum macowanii to ameliorate memory impairment induced by scopolamine. Thirty-six male Balb/c mice weighing around 25-35 g were employed in the present investigation. Y-maze and novel object recognition apparatus served as the exteroceptive behavioural models, and scopolamine-induced amnesia served as the interoceptive behavioural model. C. macowanii (10, 20, and 40 mg/kg p.o.) was administered in single doses to the mice. Donepezil (3 mg/kg p.o.) was used as a positive control agent. C. macowanii extract reversed the amnesia induced by scopolamine as indicated by a dose-dependent increase in spontaneous alternation performance in the Y-maze task. C. macowanii 40 mg/kg showed significant activity (p < 0.05 versus negative control), comparable to that of the positive control. C. macowanii also showed memory-enhancing activity against scopolamine-induced memory deficits in the long-term memory novel object recognition performance as indicated by a dose-dependent increase in the discrimination index. The results indicate that the hydroethanolic extract of C. macowanii may be a useful memory restorative mediator in the treatment of cognitive disorders such as Alzheimer's disease.
已发现马克温文殊兰含有在体外对乙酰胆碱酯酶有活性的生物碱。本研究旨在调查马克温文殊兰水乙醇粗提取物改善东莨菪碱诱导的记忆损伤的体内能力。本研究采用了36只体重约25 - 35克的雄性Balb/c小鼠。Y迷宫和新物体识别装置作为外感受性行为模型，东莨菪碱诱导的失忆作为内感受性行为模型。将马克温文殊兰（10、20和40毫克/千克，口服）单剂量给予小鼠。多奈哌齐（3毫克/千克，口服）用作阳性对照剂。如Y迷宫任务中自发交替表现的剂量依赖性增加所示，马克温文殊兰提取物逆转了东莨菪碱诱导的失忆。40毫克/千克的马克温文殊兰显示出显著活性（与阴性对照相比，p < 0.05），与阳性对照相当。如辨别指数的剂量依赖性增加所示，马克温文殊兰在长期记忆新物体识别表现中也显示出针对东莨菪碱诱导的记忆缺陷的记忆增强活性。结果表明，马克温文殊兰水乙醇提取物可能是治疗认知障碍如阿尔茨海默病的有用的记忆恢复介质。
