a University Institute of Pharmaceutical Sciences - UGC Centre of Advanced Study , Panjab University , Chandigarh 160014 , India.
Nutr Neurosci. 2018 Jun;21(5):361-372. doi: 10.1080/1028415X.2017.1291166. Epub 2017 Feb 28.
The present study was designed to evaluate the efficacy of Lactuca sativa (LS) Linn. (Asteraceae) against scopolamine-induced amnesia and to validate its traditional claim as memory enhancer.
Ethanol extract of fresh LS leaves (LSEE), standardized on the basis of quercetin content, was successively partitioned using various solvents viz., hexane, ethyl acetate, and n-butanol in increasing order of polarity. LSEE (50, 100, and 200 mg/kg) and its various fractions (at a dose equivalent to dose of LSEE exhibiting maximum activity), administered orally for 14 days, were evaluated for their memory enhancing effect against scopolamine-induced (1 mg/kg, i.p.) amnesia in 3-4 months old male Laca mice (n = 6 in each group). The memory enhancing effect was evaluated using behavioural (elevated plus maze, novel object recognition and Morris water maze tests) and biochemical parameters (acetylcholinesterase activity, malonaldehyde, superoxide dismutase, nitrite, catalase, and reduced gultathione content). The results of the test substances were compared with both scopolamine and donepezil that was used as a standard memory enhancer and acetylcholinesterase inhibitor.
Scopolamine elicit marked deterioration of memory and alteration in biochemical parameters in comparison to the control group. LSEE and its n-butanol and aqueous fractions significantly (P < 0.05) attenuated the scopolamine-induced amnesia that was evident in all the behavioural and biochemical test parameters. LSEE (200 mg/kg) and n-butanol fraction (15 mg/kg) exhibited maximum anti-amnesic effect among various tested dose levels.
The results exhibited that LS prophylaxis attenuated scopolamine-induced memory impairment through its acetylcholinesterase inhibitory and antioxidant activity validating its traditional claim.
本研究旨在评估菊苣（LS）（菊科）对东莨菪碱诱导的健忘症的疗效，并验证其作为记忆增强剂的传统说法。
以槲皮素含量为基础，对新鲜 LS 叶的乙醇提取物（LSEE）进行标准化，然后使用各种溶剂（正己烷、乙酸乙酯和正丁醇）按极性递增的顺序依次进行分区。LSEE（50、100 和 200mg/kg）及其各种馏分（剂量相当于显示最大活性的 LSEE 剂量），连续 14 天口服给药，用于评估其对 3-4 个月龄雄性 Laca 小鼠（每组 6 只）东莨菪碱（1mg/kg，ip）诱导的健忘症的记忆增强作用。使用行为（高架十字迷宫、新物体识别和 Morris 水迷宫测试）和生化参数（乙酰胆碱酯酶活性、丙二醛、超氧化物歧化酶、亚硝酸盐、过氧化氢酶和还原型谷胱甘肽含量）评估记忆增强作用。将测试物质的结果与东莨菪碱和多奈哌齐进行比较，多奈哌齐被用作标准记忆增强剂和乙酰胆碱酯酶抑制剂。
与对照组相比，东莨菪碱引起记忆明显恶化和生化参数改变。LSEE 及其正丁醇和水馏分显着（P<0.05）减弱了所有行为和生化测试参数中观察到的东莨菪碱诱导的健忘症。在各种测试剂量水平中，LSEE（200mg/kg）和正丁醇馏分（15mg/kg）表现出最大的抗健忘作用。
结果表明，LS 预防通过其乙酰胆碱酯酶抑制和抗氧化活性减弱了东莨菪碱诱导的记忆障碍，验证了其传统说法。
