Chanda Chandrasekhar, Sarkar Angshuman, Chakrabarty Dibakar
Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, K.K. Birla Goa Campus, Zuarinagar, Goa 403726, India.
Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, K.K. Birla Goa Campus, Zuarinagar, Goa 403726, India.
Arch Biochem Biophys. 2016 Jan 15;590:20-26. doi: 10.1016/j.abb.2015.11.006. Epub 2015 Nov 10.
A metalloproteinase anticoagulant toxin of molecular weight 66 kDa has been purified from the venom of Indian monocled cobra (Naja kaouthia). This toxin named as NKV 66 cleaved fibrinogen in a dose and time dependent manner. The digestion process was specific to Aα chain and cleaved fibrinogen to peptide fragments. NKV 66 completely liquefied the fibrin clots developed in vitro in 18 h. Plasma recalcification time and thrombin time were significantly prolonged following treatment of plasma with NKV 66. NKV 66 significantly inhibited ADP and collagen induced platelet aggregation in a dose dependent manner. It showed disintegrin like activity on A549 cells cultured in vitro. About 40% inhibition of adherence of A549 cells to matrix was observed following NKV 66 treatment also NKV 66 treated A549 cells were drastically inhibited from passing through the matrix in cell invasion assays in vitro, suggesting anti-adhesive properties of NKV 66.
一种分子量为66 kDa的金属蛋白酶抗凝毒素已从印度眼镜蛇（眼镜王蛇）的毒液中纯化出来。这种名为NKV 66的毒素以剂量和时间依赖性方式切割纤维蛋白原。消化过程对Aα链具有特异性，并将纤维蛋白原切割成肽片段。NKV 66在18小时内完全液化了体外形成的纤维蛋白凝块。用NKV 66处理血浆后，血浆复钙时间和凝血酶时间显著延长。NKV 66以剂量依赖性方式显著抑制ADP和胶原诱导的血小板聚集。它在体外培养的A549细胞上表现出类整合素活性。用NKV 66处理后，观察到A549细胞与基质的粘附受到约40%的抑制，在体外细胞侵袭试验中，用NKV 66处理的A549细胞通过基质的能力也受到显著抑制，这表明NKV 66具有抗粘附特性。
