蜂毒肽信使核糖核酸的体外翻译产生一种以谷氨酰胺基甘氨酸而非谷氨酰胺结尾的产物。
Translation of melittin messenger RNA in vitro yields a product terminating with glutaminylglycine rather than with glutaminamide.
作者信息
Suchanek G, Kreil G
出版信息
Proc Natl Acad Sci U S A. 1977 Mar;74(3):975-8. doi: 10.1073/pnas.74.3.975.
Abstract
Melittin messenger RNA from queen bee venom glands has been translated in a cell-free system from wheat germ. A product larger than promelittin is formed which has the carboxy-terminal sequence-Gln-Gln-GlyCOOH. Melittin and promelittin from venom glands terminate in -Gln-GlnCONH2. The possible role of the extra glycine residue in the formation of a COOH-terminal amide via a transamidase-like reaction is discussed.
摘要
来自蜂王毒腺的蜂毒肽信使核糖核酸已在小麦胚芽无细胞系统中进行了翻译。形成了一种比前蜂毒肽更大的产物，其羧基末端序列为-Gln-Gln-GlyCOOH。来自毒腺的蜂毒肽和前蜂毒肽的末端为-Gln-GlnCONH2。讨论了额外的甘氨酸残基在通过类似转酰胺酶的反应形成羧基末端酰胺中的可能作用。
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