体外合成的蜜蜂前溶血肽原的氨基酸序列。
Amino acid sequence of honeybee prepromelittin synthesized in vitro.
作者信息
Suchanek G, Kreil G, Hermodson M A
出版信息
Proc Natl Acad Sci U S A. 1978 Feb;75(2):701-4. doi: 10.1073/pnas.75.2.701.
Abstract
Translation of melittin messenger RNA from queen bee venom glands in a cell-free system from wheat germ yielded prepromelittin. Sequence analysis of the labeled in vitro product was performed by automatic Edman degradation of the intact polypeptide as well as by analysis of some of its proteolytic fragments. Prepromelittin was shown to be composed of 70 amino acids, two of which have not been identified. The sequence of melittin is located in the COOH-terminal third of the polypeptide chain (residues 44--69). Prepromelittin starts with a very hydrophobic pre-region, probably 21 residues long, followed by a pro-part of unusual sequence, containing only alanine, proline, and acidic residues. At least three post-translational reactions are required to convert prepromelittin to mellitin.
摘要
在来自小麦胚芽的无细胞体系中对蜂王毒液腺中的蜂毒明肽信使核糖核酸进行翻译，得到了前蜂毒明肽。通过对完整多肽进行自动埃德曼降解以及分析其一些蛋白水解片段，对标记的体外产物进行了序列分析。结果表明，前蜂毒明肽由70个氨基酸组成，其中有两个氨基酸尚未鉴定出来。蜂毒明肽的序列位于多肽链的羧基末端三分之一处（第44至69位氨基酸残基）。前蜂毒明肽起始于一个非常疏水的前导区，可能有21个氨基酸残基长，接着是一个具有异常序列的前肽部分，只包含丙氨酸、脯氨酸和酸性残基。将前蜂毒明肽转化为蜂毒明肽至少需要三个翻译后反应。
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1
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Nucleic Acids Res. 1974 Nov;1(11):1385-97. doi: 10.1093/nar/1.11.1385.
2
[Sequence analysis of melittin from tryptic and peptic degradation products].[胰蛋白酶和胃蛋白酶降解产物中蜂毒肽的序列分析]
Hoppe Seylers Z Physiol Chem. 1967 Jan;348(1):37-50.
3
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4
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5
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7
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