Gebregiorgis Ashenafi, Tessema Tesfaye Sisay
Semera Regional Veterinary Diagnostic Laboratory, Semera, Ethiopia.
Institute of Biotechnology, Addis Ababa University, Addis Ababa, Ethiopia.
Trop Anim Health Prod. 2016 Feb;48(2):273-81. doi: 10.1007/s11250-015-0946-9. Epub 2015 Nov 12.
This study was carried out from October 2012 to end of February 2013 in and around Kombolcha, Amhara regional state, Ethiopia, using a total of 201 neonatal calves aged 1 day to 4 months and suffering from diarrhea. The objectives of the study were to isolate Escherichia coli from diarrheic calves, and to determine E. coli biotypes and risk factors associated with its isolation. The fecal samples were collected, transported, and processed following standard microbiological procedures. Seventy-four isolates of E. coli were identified. Yellowish diarrhea, younger age, and low-colostrum feeding were significantly associated with rate of E. coli isolation (P < 0.05). Then the 74 isolates of E. coli were biotyped using fermentation of 9 sugars and grouped into 12 biotypes; the most dominant was biotype III (36.8 %). Finally, by comparing with studies elsewhere, from the 12 isolated biotypes, 3 of them were suggested to be enteropathogenic E. coli (EPEC), entherotoxigenic E. coli (ETEC), and adhesion and effacing E. coli (AEEC) pathogenic strains. The present study showed that E. coli accounted for 37 % of calf diarrhea, with very diverse biotypes.
本研究于2012年10月至2013年2月底在埃塞俄比亚阿姆哈拉州孔博尔查及其周边地区开展，共选取了201头年龄在1日龄至4个月且患有腹泻的新生犊牛。本研究的目的是从腹泻犊牛中分离出大肠杆菌，并确定大肠杆菌的生物型以及与其分离相关的风险因素。按照标准微生物学程序采集、运输和处理粪便样本。共鉴定出74株大肠杆菌。黄色腹泻、年龄较小和初乳喂养不足与大肠杆菌分离率显著相关（P < 0.05）。然后，利用9种糖的发酵对这74株大肠杆菌进行生物分型，分为12种生物型；最主要的是生物型III（36.8%）。最后，与其他地方的研究进行比较，从分离出的12种生物型中，有3种被认为是肠致病性大肠杆菌（EPEC）、产肠毒素大肠杆菌（ETEC）和黏附性侵袭性大肠杆菌（AEEC）致病菌株。本研究表明，大肠杆菌占犊牛腹泻病因的37%，生物型非常多样。
