Inoue M, Sasagawa T, Shimizu C, Shimizu H, Saito J, Ueda G, Tanizawa O
Department of Obstetrics and Gynecology, Osaka University School of Medicine, Japan.
Gynecol Oncol. 1989 Jun;33(3):344-50. doi: 10.1016/0090-8258(89)90525-8.
A murine monoclonal antibody, MCA-97, was prepared against a human endometrial adenocarcinoma cell line. In a cellular enzyme-linked immunospecific assay, the MCA-97 antibody reacted with all adenocarcinoma cell lines tested, including four endometrial adenocarcinoma lines. The antigen defined by MCA-97 was estimated to be a nonsialylated high-molecular-weight glycoprotein containing galactose and N-acetylglucosamine in its determinant. In immunoperoxidase staining of formalin-fixed, paraffin-embedded sections, MCA-97 reacted with most endometrial adenocarcinomas and ovarian endometrioid-type adenocarcinomas, and also reacted with normal glandular epithelium of the female genital and gastrointestinal tracts. However, it was mostly unreactive with squamous cell carcinoma and ovarian serous adenocarcinoma tissues. By the reversed passive hemagglutination method, the antigen defined by MCA-97 was detected in the sera of one-third of patients with endometrial adenocarcinomas, and in half of those with ovarian endometrioid adenocarcinomas. The antigen was not demonstrable in the sera of most normal female volunteers. Thus, MCA-97 monoclonal antibody has potential clinical applications in diagnostic serology and pathology.
制备了一种针对人子宫内膜腺癌细胞系的鼠单克隆抗体MCA - 97。在细胞酶联免疫特异性分析中，MCA - 97抗体与所有测试的腺癌细胞系发生反应，包括四种子宫内膜腺癌细胞系。据估计，MCA - 97所定义的抗原是一种非唾液酸化的高分子量糖蛋白，其决定簇中含有半乳糖和N - 乙酰葡糖胺。在福尔马林固定、石蜡包埋切片的免疫过氧化物酶染色中，MCA - 97与大多数子宫内膜腺癌和卵巢子宫内膜样腺癌发生反应，也与女性生殖和胃肠道的正常腺上皮发生反应。然而，它与鳞状细胞癌和卵巢浆液性腺癌组织大多无反应。通过反向被动血凝法，在三分之一的子宫内膜腺癌患者血清和一半的卵巢子宫内膜样腺癌患者血清中检测到了MCA - 97所定义的抗原。在大多数正常女性志愿者的血清中未检测到该抗原。因此，MCA - 97单克隆抗体在诊断血清学和病理学方面具有潜在的临床应用价值。
