Inoue M, Saito J, Abe Y, Tanaka Y, Ueda G, Tanizawa O
Nihon Sanka Fujinka Gakkai Zasshi. 1986 Jan;38(1):53-8.
Spleen cells from BALB/c mouse immunized with the human endometrial adenocarcinoma cell line (ISHIKAWA) were fused with mouse myeloma cell line (NS-1) in the presence of polyethylene glycol (Mr 1000). One monoclonal antibody, MCA-97 (IgM subclass), which showed reactivity with the ISHIKAWA-cell line, was obtained by a limiting dilution technique. In a cellular enzyme-linked immunospecific assay, the MCA-97 antibody reacted with all of 7 adenocarcinoma cell lines tested. In immunoperoxidase testing of formalin-fixed paraffin-embedded sections, the MCA-97 antibody reacted with most endometrial adenocarcinomas and endometrioid adenocarcinomas of the ovary, but did not react with squamous cell carcinomas or ovarian serous adenocarcinomas. In addition, it reacted with the glandular epithelium of normal tissues, such as proliferative endometrium, fallopian tube, uterine cervix and gastrointestinal tract. The reversed passive hemagglutination method demonstrated the antigen defined by MCA-97 in the sera of 5 out of 11 patients with endometrial carcinomas, and 3 out of 4 of those with ovarian endometrioid adenocarcinomas. It was not demonstrable in sera of most normal female volunteers, or any patients with cervical squamous cell carcinomas or ovarian serous adenocarcinomas. Thus, MCA-97 is of potential clinical application in diagnostic serology and pathology.
用人类子宫内膜腺癌细胞系（石川细胞系）免疫的BALB/c小鼠的脾细胞，在聚乙二醇（分子量1000）存在的情况下，与小鼠骨髓瘤细胞系（NS-1）进行融合。通过有限稀释技术获得了一种单克隆抗体MCA-97（IgM亚类），它与石川细胞系有反应性。在细胞酶联免疫特异性测定中，MCA-97抗体与所有测试的7种腺癌细胞系都有反应。在对福尔马林固定石蜡包埋切片的免疫过氧化物酶检测中，MCA-97抗体与大多数子宫内膜腺癌和卵巢子宫内膜样腺癌有反应，但与鳞状细胞癌或卵巢浆液性腺癌无反应。此外，它还与正常组织的腺上皮有反应，如增殖期子宫内膜、输卵管、子宫颈和胃肠道。反向被动血凝法显示，11例子宫内膜癌患者中有5例、4例卵巢子宫内膜样腺癌患者中有3例的血清中存在MCA-97所定义的抗原。在大多数正常女性志愿者的血清中，以及任何宫颈鳞状细胞癌或卵巢浆液性腺癌患者的血清中均未检测到该抗原。因此，MCA-97在诊断血清学和病理学方面具有潜在的临床应用价值。
