Koizumi M, Uede T, Shijubo N, Kudo R, Hashimoto M, Kikuchi K
Department of Pathology, Sapporo Medical College, Japan.
Cancer Res. 1988 Nov 15;48(22):6565-72.
A murine monoclonal antibody, 1C5, was produced by fusion of spleen cells obtained from mice immunized with CAC-1, a human cell line of adenocarcinoma derived from uterine cervix, and NS/1 myeloma cells. 1C5 can be used for the staining of routine formalin-fixed and paraffin-embedded tissue sections. 1C5-defined antigen was found to have a molecular weight of 26,000. The 1C5-defined antigen was resistant to neuraminidase and trypsin treatment, but sensitive to periodate treatment, indicating that an epitope of the 1C5-defined antigen is a carbohydrate moiety. Immunohistochemical study using immunoperoxidase staining demonstrated that 1C5 reacted with 87% of adenocarcinomas of the uterine cervix, 39% of endometrial carcinomas of the uterus, 100% of ovarian mucinous cystadenocarcinomas, 43% of ovarian serous cystadenocarcinomas, 45% of adenocarcinomas of the colon, and 40% of gastric adenocarcinomas, thus showing the broad reactivity to adenocarcinoma cells of various origins. However, 1C5 did not show any reactivity to ectocervix epithelium, cervical intraepithelial neoplasia, or squamous cell carcinoma of the uterine cervix. In addition, adenocarcinoma of the uterine cervix exhibited strong cytoplasmic reactivity with 1C5, whereas endometrial carcinoma of the uterus showed the luminal reactivity. 1C5 also reacts with 95% ethanol-fixed malignant cells in cervical smears.
一种鼠单克隆抗体1C5，是通过将用CAC - 1（一种源自子宫颈的人腺癌细胞系）免疫的小鼠的脾细胞与NS/1骨髓瘤细胞融合产生的。1C5可用于常规福尔马林固定和石蜡包埋组织切片的染色。发现1C5所识别的抗原分子量为26,000。1C5所识别的抗原对神经氨酸酶和胰蛋白酶处理具有抗性，但对高碘酸盐处理敏感，表明1C5所识别抗原的一个表位是碳水化合物部分。使用免疫过氧化物酶染色的免疫组织化学研究表明，1C5与87%的子宫颈腺癌、39%的子宫内膜癌、100%的卵巢黏液性囊腺癌、43%的卵巢浆液性囊腺癌、45%的结肠癌和40%的胃腺癌发生反应，从而显示出对各种来源的腺癌细胞具有广泛的反应性。然而，1C5对外宫颈上皮、宫颈上皮内瘤变或子宫颈鳞状细胞癌没有任何反应性。此外，子宫颈腺癌与1C5表现出强烈的细胞质反应性，而子宫内膜癌则表现出管腔反应性。1C5也与宫颈涂片中95%乙醇固定的恶性细胞发生反应。
