Metcalf D
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5327-30. doi: 10.1073/pnas.77.9.5327.
Mouse granulocyte-macrophage progenitor cells were stimulated to divide by the granulocyte-macrophage colony-stimulating factor (GM-CSF). The two daughter cells were separated; one daughter was transferred to medium containing a high concentration of GM-CSF, the other to medium containing a low concentration. Daughter cell-derived clones in the presence of 2500 units of GM-CSF had average cell cycle times 3.5 +/- 2.5 (SEM) hr shorter than clones derived from the paired daughter cell stimulated by 50 units of GM-CSF. Final colony size achieved after stimulation by 50 units of GM-CSF was always smaller than that of colonies stimulated by 2500 units of GM-CSF. In 8 of 41 instances, colonies stimulated by 50 units of GM-CSF developed, or were composed only of, macrophage populations in contrast to the granulocytic composition of colonies derived from the paired daughter cell growing in the presence of 2500 units of GM-CSF. The regulator GM-CSF appears able to directly influence cell cycle times and the pathway of differentiation entered by many bipotential granulocyte-macrophage precursor cells.
小鼠粒细胞-巨噬细胞祖细胞被粒细胞-巨噬细胞集落刺激因子（GM-CSF）刺激而分裂。将两个子细胞分离；一个子细胞转移到含有高浓度GM-CSF的培养基中，另一个转移到含有低浓度GM-CSF的培养基中。在存在2500单位GM-CSF的情况下，源自子细胞的克隆的平均细胞周期时间比由50单位GM-CSF刺激的配对子细胞衍生的克隆短3.5±2.5（SEM）小时。50单位GM-CSF刺激后最终形成的集落大小总是小于2500单位GM-CSF刺激的集落。在41个实例中的8个中，50单位GM-CSF刺激形成的集落，或者仅由巨噬细胞群体组成，这与在2500单位GM-CSF存在下生长的配对子细胞衍生的集落的粒细胞组成形成对比。调节因子GM-CSF似乎能够直接影响细胞周期时间以及许多双能粒细胞-巨噬细胞前体细胞进入的分化途径。
