选择性降低妊娠大鼠子宫灌注压的影响。

Effects of selective reduced uterine perfusion pressure in pregnant rats.

作者信息

Schenone Mauro H, Mari Giancarlo, Schlabritz-Loutsevitch Natalia, Ahokas Robert

机构信息

Department of Obstetrics and Gynecology, University of Tennessee Health Science Center, 853 Jefferson Ave, Room E102., Memphis, 38163, TN, USA.

出版信息

Placenta. 2015 Dec;36(12):1450-4. doi: 10.1016/j.placenta.2015.10.017. Epub 2015 Oct 28.

DOI:10.1016/j.placenta.2015.10.017

PMID:26565601

Abstract

INTRODUCTION

To assess the effects of selective reduced uterine perfusion pressure (SRUPP) in pregnant rats.

METHODS

20 pregnant Sprague-Dawley rats were allocated either to an intervention group, exposed to SRUPP (n = 10) or a control group, exposed to sham surgery (n = 10). Such procedures were performed on gestational day (GD) 14. The Mean arterial pressure (MAP) was measured on GD14 (before surgery) and GD20. We measured 18 h proteinuria on GD20. On GD21, mean fetal (MFW) and placental (MPW) weights were obtained. Oxidative stress and angiogenic markers were measured in placental tissue and urine. Mann Whitney U or Independent samples T test were used when appropriate. A two-sided P < 0.05 indicated statistical significance.

RESULTS

MAP on GD20 was higher in the intervention group (109 ± 1.7 mmHg) when compared with the control group (83 ± 1.5 mmHg) (P = 0.002). There was no significant difference in urinary protein excretion (117 ± 3.1 mg/24 h versus 136 mg ± 2.8/24 h, P = 0.18), MFW (4.14 ± 0.05 versus 4.39 ± 0.04 g, P = 0.19) or MPW (0.43 ± 0.008 versus 0.44 ± 0.006 g, P = 0.73) between the intervention and the control groups, respectively. The oxidative stress was increased; whereas, the sFLT1 expression was not increased when the SRUPP group was compared with controls.

DISCUSSION

SRUPP is associated with an increase in maternal MAP and oxidative stress and therefore it may become a useful tool in the study of pregnancy-related hypertensive disorders.

摘要

引言

评估选择性降低子宫灌注压（SRUPP）对妊娠大鼠的影响。

方法

将20只妊娠的Sprague-Dawley大鼠分为干预组（n = 10），使其暴露于SRUPP，或对照组（n = 10），进行假手术。这些操作在妊娠第14天（GD14）进行。在GD14（手术前）和GD20测量平均动脉压（MAP）。在GD20测量18小时蛋白尿。在GD21，获取平均胎儿体重（MFW）和胎盘重量（MPW）。测量胎盘组织和尿液中的氧化应激和血管生成标志物。在适当的时候使用曼-惠特尼U检验或独立样本t检验。双侧P < 0.05表示具有统计学意义。

结果

与对照组（83 ± 1.5 mmHg）相比，干预组在GD20时的MAP更高（109 ± 1.7 mmHg）（P = 0.002）。干预组和对照组之间的尿蛋白排泄量（117 ± 3.1 mg/24 h对136 mg ± 2.8/24 h，P = 0.18）、MFW（4.14 ± 0.05对4.39 ± 0.04 g，P = 0.19）或MPW（0.43 ± 0.008对0.44 ± 0.006 g，P = 0.73）没有显著差异。与对照组相比，SRUPP组的氧化应激增加；然而，sFLT1表达没有增加。